spectrin β III Antibody (A-8): sc-515737

spectrin β III Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects spectrin beta III protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-spectrin β III antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spectrin beta III plays a crucial role in maintaining the structural integrity of the cell by forming part of the cytoskeletal framework, which is essential for cellular shape, stability, and organization. Spectrin beta III is predominantly located in the cytoplasm and is a major component of the Golgi and vesicular membrane skeletons, where spectrin beta III contributes to the organization of membrane domains and the regulation of intracellular transport. The proper localization and function of spectrin beta III are vital for cellular processes such as vesicle trafficking and signal transduction, making spectrin beta III an important target for studies related to cellular dynamics and pathology. Notably, spectrin beta III is highly expressed in the brain, kidney, pancreas, and liver, with lower expression levels in the lung and placenta, highlighting spectrin beta III′s significance in various physiological contexts. Western blotting (WB) analysis indicates that spectrin beta III migrates at a higher molecular mass than predicted in the kidney, which may reflect spectrin beta III′s complex post-translational modifications and interactions with other proteins, further underscoring spectrin beta III′s importance in cellular function and integrity.

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spectrin β III Antibody (A-8) References:

1. beta III spectrin binds to the Arp1 subunit of dynactin.  |  Holleran, EA., et al. 2001. J Biol Chem. 276: 36598-605. PMID: 11461920
2. Evolution of spectrin function in cytoskeletal and membrane networks.  |  Baines, AJ. 2009. Biochem Soc Trans. 37: 796-803. PMID: 19614597
3. Loss of beta-III spectrin leads to Purkinje cell dysfunction recapitulating the behavior and neuropathology of spinocerebellar ataxia type 5 in humans.  |  Perkins, EM., et al. 2010. J Neurosci. 30: 4857-67. PMID: 20371805
4. Mutant β-III spectrin causes mGluR1α mislocalization and functional deficits in a mouse model of spinocerebellar ataxia type 5.  |  Armbrust, KR., et al. 2014. J Neurosci. 34: 9891-904. PMID: 25057192
5. Functional diversity among spectrin isoforms.  |  Coleman, TR., et al. 1989. Cell Motil Cytoskeleton. 12: 225-47. PMID: 2655937
6. The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis.  |  Saxton, MJ. 1989. Biophys J. 55: 21-8. PMID: 2930822
7. β-III-spectrin immunohistochemistry as a potential diagnostic tool with high sensitivity for malignant peripheral nerve sheath tumors.  |  Hirbe, AC., et al. 2018. Neuro Oncol. 20: 858-860. PMID: 29596596
8. The Spread of Spectrin in Ataxia and Neurodegenerative Disease.  |  Morrow, JS. and Stankewich, MC. 2021. J Exp Neurol. 2: 131-139. PMID: 34528024
9. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins.  |  Speicher, DW. 1986. J Cell Biochem. 30: 245-58. PMID: 3517024
10. Modulation of spectrin-actin assembly by erythrocyte adducin.  |  Gardner, K. and Bennett, V. Nature. 328: 359-62. PMID: 3600811
11. A partial structural repeat forms the heterodimer self-association site of all beta-spectrins.  |  Kennedy, SP., et al. 1994. J Biol Chem. 269: 11400-8. PMID: 8157672
12. A widely expressed betaIII spectrin associated with Golgi and cytoplasmic vesicles.  |  Stankewich, MC., et al. 1998. Proc Natl Acad Sci U S A. 95: 14158-63. PMID: 9826670

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-spectrin β III Antibody (A-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-spectrin β III Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

spectrin β III Antibody (A-8) Specifications

Alternate Product Name: spectrin β III

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-22

Epitope Target: spectrin β III

Epitope Species: human

Product Reactivity/Detection: spectrin β III

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for spectrin β III Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin