spectrin Antibody (RG/26): sc-53444

spectrin Antibody (RG/26) is a mouse monoclonal IgG2a kappa light chain antibody that detects spectrin from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-spectrin antibody (RG/26) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spectrin plays a crucial role in maintaining plasma membrane structural integrity, particularly in erythrocytes, where spectrin forms a vital component of the cytoskeletal framework. Spectrin organizes into a network providing mechanical support and flexibility to the cell membrane, allowing red blood cells to deform as they navigate through narrow capillaries. The spectrin cytoskeleton consists of nonhomologous α and β chains that assemble into dimers, tetramers, and higher-order polymers, essential for membrane shape and stability. Notably, spectrin α I and spectrin β I are predominantly found in erythrocytes, while spectrin α II and spectrin β II exist in other somatic cells, highlighting spectrin′s diverse roles across different cell types. Spectrin′s dynamic assembly and disassembly is critical for cellular processes, and spectrin′s interaction with other cytoskeletal proteins ensures proper cell membrane function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

spectrin Antibody (RG/26) References:

1. Selective release of calpain produced alphalI-spectrin (alpha-fodrin) breakdown products by acute neuronal cell death.  |  Dutta, S., et al. 2002. Biol Chem. 383: 785-91. PMID: 12108543
2. Erythroid expression of the human alpha-spectrin gene promoter is mediated by GATA-1- and NF-E2-binding proteins.  |  Boulanger, L., et al. 2002. J Biol Chem. 277: 41563-70. PMID: 12196550
3. Functional diversity among spectrin isoforms.  |  Coleman, TR., et al. 1989. Cell Motil Cytoskeleton. 12: 225-47. PMID: 2655937
4. The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis.  |  Saxton, MJ. 1989. Biophys J. 55: 21-8. PMID: 2930822
5. Comparison of nonerythroid alpha-spectrin genes reveals strict homology among diverse species.  |  Leto, TL., et al. 1988. Mol Cell Biol. 8: 1-9. PMID: 3336352
6. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins.  |  Speicher, DW. 1986. J Cell Biochem. 30: 245-58. PMID: 3517024
7. Modulation of spectrin-actin assembly by erythrocyte adducin.  |  Gardner, K. and Bennett, V. Nature. 328: 359-62. PMID: 3600811
8. A partial structural repeat forms the heterodimer self-association site of all beta-spectrins.  |  Kennedy, SP., et al. 1994. J Biol Chem. 269: 11400-8. PMID: 8157672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-spectrin Antibody (RG/26) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-spectrin Antibody (RG/26) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

spectrin Antibody (RG/26) Specifications

Alternate Product Name: spectrin

Product Clone Name: RG/26

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: spectrin

Epitope Species: human

Product Reactivity/Detection: spectrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for spectrin Antibody (RG/26): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin