spectrin α I Antibody (B-12): sc-271130
- spectrin α I Antibody (B-12) is a mouse monoclonal IgG1 κ spectrin α I antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 541-650 of spectrin α I of human origin
- spectrin alpha I Antibody (B-12) is recommended for detection of spectrin α I of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-spectrin alpha I Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for spectrin α I Antibody (B-12) for WB and IHC(P) applications. These reagents are now offered in bundles with spectrin α I Antibody (B-12) (see ordering information below).
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spectrin α I Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects spectrin alpha I protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-spectrin α I antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. spectrin alpha I plays a crucial role in maintaining the integrity and stability of erythrocyte plasma membrane, as spectrin alpha I is a key component of the cytoskeletal framework that determines cell shape and mechanical properties. spectrin alpha I is primarily located beneath the plasma membrane of erythrocytes, where spectrin alpha I forms a mesh-like structure that supports the membrane and facilitates flexibility during deformation. This structural organization is vital for red blood cells as they navigate through narrow capillaries, ensuring efficient oxygen transport throughout the body. Additionally, spectrin alpha I interacts with other cytoskeletal proteins, such as actin and band 3, to form a dynamic network that is essential for cellular stability and function. Defects in spectrin alpha I can lead to various red blood cell disorders, including hereditary spherocytosis and elliptocytosis, highlighting the importance of spectrin alpha I in maintaining normal erythrocyte morphology and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
spectrin α I Antibody (B-12) References:
- Erythroid expression of the human alpha-spectrin gene promoter is mediated by GATA-1- and NF-E2-binding proteins. | Boulanger, L., et al. 2002. J Biol Chem. 277: 41563-70. PMID: 12196550
- Functional diversity among spectrin isoforms. | Coleman, TR., et al. 1989. Cell Motil Cytoskeleton. 12: 225-47. PMID: 2655937
- The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis. | Saxton, MJ. 1989. Biophys J. 55: 21-8. PMID: 2930822
- The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins. | Speicher, DW. 1986. J Cell Biochem. 30: 245-58. PMID: 3517024
- Modulation of spectrin-actin assembly by erythrocyte adducin. | Gardner, K. and Bennett, V. Nature. 328: 359-62. PMID: 3600811
- A partial structural repeat forms the heterodimer self-association site of all beta-spectrins. | Kennedy, SP., et al. 1994. J Biol Chem. 269: 11400-8. PMID: 8157672
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
spectrin α I Antibody (B-12) | sc-271130 | 200 µg/ml | $322.00 | |||
spectrin α I Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-527209 | 200 µg Ab; 10 µg BP | $361.00 | |||
spectrin α I Antibody (B-12): m-IgG1 BP-HRP Bundle | sc-532582 | 200 µg Ab; 20 µg BP | $361.00 | |||
spectrin α I Antibody (B-12) AC | sc-271130 AC | 500 µg/ml, 25% agarose | $424.00 | |||
spectrin α I Antibody (B-12) HRP | sc-271130 HRP | 200 µg/ml | $322.00 | |||
spectrin α I Antibody (B-12) FITC | sc-271130 FITC | 200 µg/ml | $336.00 | |||
spectrin α I Antibody (B-12) PE | sc-271130 PE | 200 µg/ml | $349.00 | |||
spectrin α I Antibody (B-12) Alexa Fluor® 488 | sc-271130 AF488 | 200 µg/ml | $364.00 | |||
spectrin Antibody (B-12) Alexa Fluor® 546 | sc-271130 AF546 | 200 µg/ml | $364.00 | |||
spectrin α I Antibody (B-12) Alexa Fluor® 594 | sc-271130 AF594 | 200 µg/ml | $364.00 | |||
spectrin α I Antibody (B-12) Alexa Fluor® 647 | sc-271130 AF647 | 200 µg/ml | $364.00 | |||
spectrin α I Antibody (B-12) Alexa Fluor® 680 | sc-271130 AF680 | 200 µg/ml | $364.00 | |||
spectrin α I Antibody (B-12) Alexa Fluor® 790 | sc-271130 AF790 | 200 µg/ml | $364.00 |