SPATA16 Antibody (G-2): sc-374112
- SPATA16 Antibody (G-2) is a mouse monoclonal IgG2a κ SPATA16 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 549-569 at the C-terminus of SPATA16 of human origin
- SPATA16 Antibody (G-2) is recommended for detection of SPATA16 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SPATA16 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPATA16 Antibody (G-2) for WB and IHC(P) applications. These reagents are now offered in bundles with SPATA16 Antibody (G-2) (see ordering information below).
SPATA16 Antibody (G-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects SPATA16 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SPATA16 antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPATA16, also known as spermatogenesis associated 16 or NYD-SP12, is a 569 amino acid protein that plays a crucial role in sperm acrosome development, essential for sperm-egg fusion during fertilization. SPATA16 is primarily localized in the Golgi apparatus and predominantly expressed in the testis, with lower expression levels found in the kidney and pancreas. SPATA16 gene is located on human chromosome 3q26.31 and mouse chromosome 3 A3, and mutations in SPATA16 can lead to globozoospermia, a rare form of teratozoospermia characterized by abnormal sperm morphology and infertility. Understanding SPATA16 function is vital, as SPATA16 not only contributes to normal spermatogenesis but also has implications for male fertility, making SPATA16 a significant target for research in reproductive biology and potential therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPATA16 Antibody (G-2) References:
- Identification and characterization of a novel human testis-specific Golgi protein, NYD-SP12. | Xu, M., et al. 2003. Mol Hum Reprod. 9: 9-17. PMID: 12529416
- Chromosome abnormalities in sperm from infertile men with normal somatic karyotypes: teratozoospermia. | Machev, N., et al. 2005. Cytogenet Genome Res. 111: 352-7. PMID: 16192715
- Gene functional research using polyethylenimine-mediated in vivo gene transfection into mouse spermatogenic cells. | Lu, L., et al. 2006. Asian J Androl. 8: 53-9. PMID: 16372119
- Rapid evolution, genetic variations, and functional association of the human spermatogenesis-related gene NYD-SP12. | Zhang, Q., et al. 2007. J Mol Evol. 65: 154-61. PMID: 17665087
- Homozygous mutation in SPATA16 is associated with male infertility in human globozoospermia. | Dam, AH., et al. 2007. Am J Hum Genet. 81: 813-20. PMID: 17847006
- On round-headed human spermatozoa. | Kullander, S. and Rausing, A. 1975. Int J Fertil. 20: 33-40. PMID: 4383
- Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm. | Battaglia, DE., et al. 1997. Fertil Steril. 68: 118-22. PMID: 9207595
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPATA16 Antibody (G-2) | sc-374112 | 200 µg/ml | $316.00 | |||
SPATA16 Antibody (G-2): m-IgG Fc BP-HRP Bundle | sc-529664 | 200 µg Ab; 10 µg BP | $354.00 | |||
SPATA16 Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-522626 | 200 µg Ab, 40 µg BP | $354.00 | |||
SPATA16 Antibody (G-2): m-IgG2a BP-HRP Bundle | sc-547355 | 200 µg Ab; 10 µg BP | $354.00 | |||
SPATA16 Antibody (G-2) AC | sc-374112 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SPATA16 Antibody (G-2) HRP | sc-374112 HRP | 200 µg/ml | $316.00 | |||
SPATA16 Antibody (G-2) FITC | sc-374112 FITC | 200 µg/ml | $330.00 | |||
SPATA16 Antibody (G-2) PE | sc-374112 PE | 200 µg/ml | $343.00 | |||
SPATA16 Antibody (G-2) Alexa Fluor® 488 | sc-374112 AF488 | 200 µg/ml | $357.00 | |||
SPATA16 Antibody (G-2) Alexa Fluor® 546 | sc-374112 AF546 | 200 µg/ml | $357.00 | |||
SPATA16 Antibody (G-2) Alexa Fluor® 594 | sc-374112 AF594 | 200 µg/ml | $357.00 | |||
SPATA16 Antibody (G-2) Alexa Fluor® 647 | sc-374112 AF647 | 200 µg/ml | $357.00 | |||
SPATA16 Antibody (G-2) Alexa Fluor® 680 | sc-374112 AF680 | 200 µg/ml | $357.00 | |||
SPATA16 Antibody (G-2) Alexa Fluor® 790 | sc-374112 AF790 | 200 µg/ml | $357.00 | |||
SPATA16 (G-2) Neutralizing Peptide | sc-374112 P | 100 µg/0.5 ml | $68.00 |