Sp3 Antibody (F-11): sc-55479

Sp3 Antibody (F-11) is a mouse monoclonal IgG2a light chain antibody that detects Sp3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sp3 is a member of the Sp transcription factor family, which also includes Sp1, Sp2, and Sp4, and is characterized by a highly conserved DNA-binding domain featuring three zinc fingers at its C-terminus. This structural feature allows Sp3 to bind to specific DNA sequences known as GC boxes, which are essential for the regulation of gene expression. Sp3 plays a unique role in transcriptional regulation of various genes by inhibiting the activity of other Sp family members, such as Sp1 and Sp4, thereby fine-tuning the transcriptional response in different cellular contexts. Sp3′s interaction with regulatory elements, such as the E2 element for the β4 subunit of neuronal nicotinic acetylcholine receptors, highlights Sp3′s importance in neuronal signaling and development. Multiple isoforms of Sp3, generated through alternative splicing, add another layer of complexity to Sp3′s functional repertoire, making Sp3 a critical player in cellular processes and a valuable target for research in gene regulation and neurobiology.

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Sp3 Antibody (F-11) References:

1. Gene regulation by Sp1 and Sp3.  |  Li, L., et al. 2004. Biochem Cell Biol. 82: 460-71. PMID: 15284899
2. Regulation of the dual-function transcription factor Sp3 by SUMO.  |  Valin, A. and Gill, G. 2007. Biochem Soc Trans. 35: 1393-6. PMID: 18031229
3. The role of Sp1 and Sp3 in normal and cancer cell biology.  |  Li, L. and Davie, JR. 2010. Ann Anat. 192: 275-83. PMID: 20810260
4. Transcriptional regulation of BNIP3 by Sp3 in prostate cancer.  |  Huang, Y., et al. 2015. Prostate. 75: 1556-67. PMID: 26012884
5. The miR-491-3p/Sp3/ABCB1 axis attenuates multidrug resistance of hepatocellular carcinoma.  |  Zhao, Y., et al. 2017. Cancer Lett. 408: 102-111. PMID: 28844709
6. Solvent Precipitation SP3 (SP4) Enhances Recovery for Proteomics Sample Preparation without Magnetic Beads.  |  Johnston, HE., et al. 2022. Anal Chem. 94: 10320-10328. PMID: 35848328
7. SP3-FAIMS-Enabled High-Throughput Quantitative Profiling of the Cysteinome.  |  Desai, HS., et al. 2022. Curr Protoc. 2: e492. PMID: 35895291
8. Transcriptional activation of CSTB gene expression by transcription factor Sp3.  |  Zhu, W., et al. 2023. Biochem Biophys Res Commun. 649: 71-78. PMID: 36745972
9. Lectin-Based SP3 Technology Enables N-Glycoproteomic Analysis of Mouse Oocytes.  |  Huo, Z., et al. 2024. J Proteome Res. 23: 2137-2147. PMID: 38787631
10. Sp3 encodes multiple proteins that differ in their capacity to stimulate or repress transcription.  |  Kennett, SB., et al. 1997. Nucleic Acids Res. 25: 3110-7. PMID: 9224612

The preferred Sp3 antibody is Sp3 Antibody (F-7): sc-28305.