Sp2 Antibody (A-8): sc-17814
- Sp2 Antibody (A-8) is a mouse monoclonal IgG1 κ Sp2 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 154-435 of Sp2 of human origin
- Sp2 Antibody (A-8) is recommended for detection of Sp2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Sp2 Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-17814 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Sp2 Antibody (A-8) for WB applications. These reagents are now offered in bundles with Sp2 Antibody (A-8) (see ordering information below).
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Sp2 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sp2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sp2 (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sp2 protein, a member of the Sp transcription factor family, plays a crucial role in regulating gene expression by binding to specific DNA sequences known as GC boxes, although Sp2 exhibits a weaker affinity for GT boxes compared to its family counterparts. This differential binding is significant as Sp2 influences the transcriptional activity of genes involved in various cellular processes, including cell growth and differentiation. Sp transcription factors, which also include Sp1, Sp3, and Sp4, share a conserved DNA-binding domain characterized by three zinc fingers at the C-terminus, yet each performs distinct functions within the cell. While Sp1, Sp3, and Sp4 are ubiquitously expressed, Sp2′s expression is more restricted, which may contribute to unique regulatory roles in specific tissues. The gene encoding human Sp2 is located on chromosome 17p32.3, highlighting Sp2′s genetic significance in the context of human health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sp2 Antibody (A-8) References:
- Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. | Kingsley, C. and Winoto, A. 1992. Mol Cell Biol. 12: 4251-61. PMID: 1341900
- Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. | Hagen, G., et al. 1992. Nucleic Acids Res. 20: 5519-25. PMID: 1454515
- Identification of Sp2 as a transcriptional repressor of carcinoembryonic antigen-related cell adhesion molecule 1 in tumorigenesis. | Phan, D., et al. 2004. Cancer Res. 64: 3072-8. PMID: 15126343
- Sp2 localizes to subnuclear foci associated with the nuclear matrix. | Moorefield, KS., et al. 2006. Mol Biol Cell. 17: 1711-22. PMID: 16467376
- Sp2 promotes invasion and metastasis of hepatocellular carcinoma by targeting TRIB3 protein. | Zhu, Y., et al. 2020. Cancer Med. 9: 3592-3603. PMID: 32160655
- Transcription factor Sp2 promotes TGFB-mediated interstitial cell osteogenic differentiation in bicuspid aortic valves through a SMAD-dependent pathway. | Zheng, R., et al. 2022. Exp Cell Res. 411: 112972. PMID: 34914964
- Csp2-H Amination Reactions Mediated by Metastable Pseudo-Oh Masked Aryl-CoIII-nitrene Species. | Capdevila, L., et al. 2022. Inorg Chem. 61: 14075-14085. PMID: 35997604
- Organocatalytic Csp2-O Amination of Quinolin-4(1H)-ones with 3-Alkynyl-3-hydroxyisoindolinones. | Huang, T., et al. 2024. J Org Chem. 89: 9086-9091. PMID: 38815157
- Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3. | Hagen, G., et al. 1995. J Biol Chem. 270: 24989-94. PMID: 7559627
- Sp1-mediated transcriptional activation is repressed by Sp3. | Hagen, G., et al. 1994. EMBO J. 13: 3843-51. PMID: 8070411
- Sp1 and Sp3 regulate expression of the neuronal nicotinic acetylcholine receptor beta4 subunit gene. | Bigger, CB., et al. 1997. J Biol Chem. 272: 25976-82. PMID: 9325332
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sp2 Antibody (A-8) | sc-17814 | 200 µg/ml | $322.00 | |||
Sp2 Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-526574 | 200 µg Ab; 10 µg BP | $361.00 | |||
Sp2 Antibody (A-8): m-IgG1 BP-HRP Bundle | sc-531947 | 200 µg Ab; 20 µg BP | $361.00 | |||
Sp2 Antibody (A-8) X | sc-17814 X | 200 µg/0.1 ml | $322.00 | |||
Sp2 Antibody (A-8) AC | sc-17814 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Sp2 Antibody (A-8) HRP | sc-17814 HRP | 200 µg/ml | $322.00 | |||
Sp2 Antibody (A-8) FITC | sc-17814 FITC | 200 µg/ml | $336.00 | |||
Sp2 Antibody (A-8) PE | sc-17814 PE | 200 µg/ml | $349.00 | |||
Sp2 Antibody (A-8) Alexa Fluor® 488 | sc-17814 AF488 | 200 µg/ml | $364.00 | |||
Sp2 Antibody (A-8) Alexa Fluor® 546 | sc-17814 AF546 | 200 µg/ml | $364.00 | |||
Sp2 Antibody (A-8) Alexa Fluor® 594 | sc-17814 AF594 | 200 µg/ml | $364.00 | |||
Sp2 Antibody (A-8) Alexa Fluor® 647 | sc-17814 AF647 | 200 µg/ml | $364.00 | |||
Sp2 Antibody (A-8) Alexa Fluor® 680 | sc-17814 AF680 | 200 µg/ml | $364.00 | |||
Sp2 Antibody (A-8) Alexa Fluor® 790 | sc-17814 AF790 | 200 µg/ml | $364.00 |