Sos 2 Antibody (B-6): sc-393667

Sos 2 (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sos 2 protein in mouse, rat and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Sos 2 antibody (B-6) is available unconjugated and in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and various Alexa Fluor® conjugates. The superfamily of GTP-binding proteins, including Ras proteins as prototypes, plays roles in diverse biological activities. Research has identified guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an "adapter" protein (Sem 5/GRB2) that mediates GRF interactions with activated receptor molecules. Ras-GRF p140 promotes nucleotide exchange specifically on Ras p21s but not other Ras gene superfamily members. Three mammalian homologs of Drosophila Ras-GRF, son of sevenless (Sos), have been identified - mSos 1 and mSos 2 from mouse, and hSos from human. Vav p95 functions as a GRF in Ras activation by T cell receptor and contains a domain similar to Dbl p115, a GRF specific for Cdc42Hs. Following activation, Ras interacts with Raf to activate the MAP kinase phosphorylation pathway.

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Sos 2 Antibody (B-6) References:

1. Ras-guanine nucleotide exchange factor sos2 is dispensable for mouse growth and development.  |  Esteban, LM., et al. 2000. Mol Cell Biol. 20: 6410-3. PMID: 10938118
2. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
3. Activating Mutations Affecting the Dbl Homology Domain of SOS2 Cause Noonan Syndrome.  |  Cordeddu, V., et al. 2015. Hum Mutat. 36: 1080-7. PMID: 26173643
4. Oncogenic RAS isoforms show a hierarchical requirement for the guanine nucleotide exchange factor SOS2 to mediate cell transformation.  |  Sheffels, E., et al. 2018. Sci Signal. 11: PMID: 30181243
5. Variants of SOS2 are a rare cause of Noonan syndrome with particular predisposition for lymphatic complications.  |  Lissewski, C., et al. 2021. Eur J Hum Genet. 29: 51-60. PMID: 32788663
6. SOS2 modulates the threshold of EGFR signaling to regulate osimertinib efficacy and resistance in lung adenocarcinoma.  |  Theard, PL., et al. 2024. Mol Oncol. 18: 641-661. PMID: 38073064
7. The function of GRB2 in linking the insulin receptor to Ras signaling pathways.  |  Skolnik, EY., et al. 1993. Science. 260: 1953-5. PMID: 8316835
8. Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1.  |  Zhang, XF., et al. 1993. Nature. 364: 308-13. PMID: 8332187
9. An SH3-SH2-SH3 protein is required for p21Ras1 activation and binds to sevenless and Sos proteins in vitro.  |  Simon, MA., et al. 1993. Cell. 73: 169-77. PMID: 8462097
10. Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation.  |  Egan, SE., et al. 1993. Nature. 363: 45-51. PMID: 8479536
11. Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor.  |  Buday, L. and Downward, J. 1993. Cell. 73: 611-20. PMID: 8490966
12. Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2.  |  Chardin, P., et al. 1993. Science. 260: 1338-43. PMID: 8493579

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sos 2 Antibody (B-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Sos 2 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sos 2 Antibody (B-6) Specifications

Alternate Product Name: Sos 2

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1268-1297

Epitope Target: Sos 2

Epitope Species: mouse

Product Reactivity/Detection: Sos 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Sos 2 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin