SO Antibody (F-6): sc-393688
- SO Antibody (F-6) is a mouse monoclonal IgG2b κ SO antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 110-127 near the N-terminus of SO of human origin
- SO Antibody (F-6) is recommended for detection of precursor and mature SO of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SO Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SO Antibody (F-6) for WB applications. These reagents are now offered in bundles with SO Antibody (F-6) (see ordering information below).
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SEE ALSO...
SO Antibody (F-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects SO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SO (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sulfite oxidase (SO) is a crucial homodimeric enzyme located in the intermembrane space of mitochondria, where SO catalyzes the oxidation of sulfite to sulfate, representing the final step in the oxidative degradation of sulfur-containing amino acids such as cysteine and methionine. SO deficiency can lead to severe neurological disorders and early mortality. Mutations in the Arginine 160 residue in humans significantly impair the intramolecular electron transfer rate, exacerbating the severity of related genetic disorders. The tyrosine 343 residue is essential for substrate binding and the oxidation process, highlighting the intricate structure-function relationship of SO. The human SO gene is located on chromosome 12 and exhibits high expression levels in critical tissues, including the liver, kidney, skeletal muscle, heart, placenta, and brain, underscoring SO importance in various physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SO Antibody (F-6) References:
- Essential role of conserved arginine 160 in intramolecular electron transfer in human sulfite oxidase. | Feng, C., et al. 2003. Biochemistry. 42: 12235-42. PMID: 14567685
- The role of tyrosine 343 in substrate binding and catalysis by human sulfite oxidase. | Wilson, HL. and Rajagopalan, KV. 2004. J Biol Chem. 279: 15105-13. PMID: 14729666
- New approaches towards laboratory diagnosis of isolated sulphite oxidase deficiency. | Sass, JO., et al. 2004. Ann Clin Biochem. 41: 157-9. PMID: 15025809
- Effect of sulfite on cognitive function in normal and sulfite oxidase deficient rats. | Küçükatay, V., et al. 2005. Neurotoxicol Teratol. 27: 47-54. PMID: 15681120
- Effect of sulfite on macrophage functions of normal and sulfite oxidase-deficient rats. | Izgüt-Uysal, VN., et al. 2005. Food Chem Toxicol. 43: 599-605. PMID: 15721208
- Molecular basis of sulfite oxidase deficiency from the structure of sulfite oxidase. | Kisker, C., et al. 1997. Cell. 91: 973-83. PMID: 9428520
- Human sulfite oxidase R160Q: identification of the mutation in a sulfite oxidase-deficient patient and expression and characterization of the mutant enzyme. | Garrett, RM., et al. 1998. Proc Natl Acad Sci U S A. 95: 6394-8. PMID: 9600976
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SO Antibody (F-6) | sc-393688 | 200 µg/ml | $322.00 | |||
SO Antibody (F-6): m-IgG Fc BP-HRP Bundle | sc-530540 | 200 µg Ab; 10 µg BP | $361.00 | |||
SO Antibody (F-6): m-IgGκ BP-HRP Bundle | sc-524046 | 200 µg Ab, 40 µg BP | $361.00 | |||
SO Antibody (F-6): m-IgG2b BP-HRP Bundle | sc-550196 | 200 µg Ab; 10 µg BP | $361.00 | |||
SO Antibody (F-6) AC | sc-393688 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SO Antibody (F-6) HRP | sc-393688 HRP | 200 µg/ml | $322.00 | |||
SO Antibody (F-6) FITC | sc-393688 FITC | 200 µg/ml | $336.00 | |||
SO Antibody (F-6) PE | sc-393688 PE | 200 µg/ml | $349.00 | |||
SO Antibody (F-6) Alexa Fluor® 488 | sc-393688 AF488 | 200 µg/ml | $364.00 | |||
SO Antibody (F-6) Alexa Fluor® 546 | sc-393688 AF546 | 200 µg/ml | $364.00 | |||
SO Antibody (F-6) Alexa Fluor® 594 | sc-393688 AF594 | 200 µg/ml | $364.00 | |||
SO Antibody (F-6) Alexa Fluor® 647 | sc-393688 AF647 | 200 µg/ml | $364.00 | |||
SO Antibody (F-6) Alexa Fluor® 680 | sc-393688 AF680 | 200 µg/ml | $364.00 | |||
SO Antibody (F-6) Alexa Fluor® 790 | sc-393688 AF790 | 200 µg/ml | $364.00 | |||
SO (F-6) Neutralizing Peptide | sc-393688 P | 100 µg/0.5 ml | $69.00 |