SNX4 Antibody (H-8) recommended for detection of SNX4 of mouse, rat and human origin by WB, IP, IF and ELISA

SNX4 Antibody (H-8): sc-393954

SNX4 Antibody (H-8) is rated 5.0 out of 5 by 1.
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Datasheets

    • SNX4 Antibody (H-8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 151-450 mapping at the C-terminus of SNX4 of human origin
    • recommended for detection of SNX4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

How much sample do you recommend loading on the gel for Western blot detection using SNX4 (H-8): sc-393954?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Very good Western blot data of SNX4 expression Very good Western blot data of SNX4 expression in Jurkat and HeLa whole cell lysates. -SCBT QC
Date published: 2013-11-08
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