SNX4 Antibody (B-4) recommended for detection of SNX4 of mouse, rat and human origin by WB, IP, IF and ELISA

SNX4 Antibody (B-4): sc-271147

SNX4 Antibody (B-4) is rated 4.2 out of 5 by 5.
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Datasheets

    • Anti-SNX4 Antibody (B-4) is a mouse monoclonal IgG2a SNX4 antibody provided at 200 µg/ml
    • raised against a peptide mapping within an internal region of SNX4 of human origin
    • Anti-SNX4 Antibody (B-4) is recommended for detection of SNX4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine
    • Anti-SNX4 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SNX4 (B-4): sc-271147.

What application is the blocking peptide sc-271147 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from specific band by westernblot Great antibody for westernblot. Used at 1/200, specificity of the band was confirmed by SNX4 knockdown in MEF cells.
Date published: 2018-05-17
Rated 1 out of 5 by from does not work for IF tried form fix or methanol fix, 2 per cent bsa blocking, barely any signal.
Date published: 2018-03-31
Rated 5 out of 5 by from Excellent WB band! Excellent WB band in mouse cardiomyocytes of 1:1000 dilution.
Date published: 2017-06-28
Rated 5 out of 5 by from Great Western Blot data of SNX4 expression in non Great Western Blot data of SNX4 expression in non-transfected and mouse SNX4 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Nice Western Blot data of SNX4 expression Nice Western Blot data of SNX4 expression in Jurkat, HeLa, A-431, Hep G2 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-09-21
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