Anti-SNX27 Antibody (F-2) recommended for detection of SNX27 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-SNX27 Antibody (F-2): sc-515707

Anti-SNX27 Antibody (F-2) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-SNX27 Antibody (F-2) is a mouse monoclonal IgG2a (kappa light chain) SNX27 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 449-466 near the C-terminus of SNX27 of human origin
    • Anti-SNX27 Antibody (F-2) is recommended for detection of SNX27 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SNX27 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SNX27 (F-2): sc-515707.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SNX27 Antibody (F-2). This work is in progress.
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How well does the Anti-SNX27 Antibody (F-2): sc-515707 work for IHC(P) as there is no information for it on the datasheet?

Asked by: Hann
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2022-01-12

What is the recommended starting dilution for Western blot for SNX27 (F-2): sc-515707 monoclonal antibody?

Asked by: TinTin
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-515707.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Didn't work for me I used this AB for WesternBlots with MCF7 whole cell lysate Poor signal detected by ECL, several bands, nothing specific. Not better with high sensitivity detection reagent : more bands but nothing specific.
Date published: 2020-07-31
Rated 5 out of 5 by from Nice Strong bands in WB with NIH/3T3 and 3T3-L1 whole cell lysates,clean,nice.
Date published: 2017-01-05
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