SNX27 Antibody (F-2): sc-515707
- SNX27 Antibody (F-2) is a mouse monoclonal IgG2a κ SNX27 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 449-466 near the C-terminus of SNX27 of human origin
- SNX27 Antibody (F-2) is recommended for detection of SNX27 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SNX27 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SNX27 Antibody (F-2). This work is in progress.
SNX27 Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects SNX27 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SNX27 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SNX27, consisting of 541 amino acids, plays a crucial role in intracellular trafficking and recycling pathways, primarily localized in the cytoplasm and early endosomes. SNX27 features a characteristic phox homology (PX) domain, a Ras-associating domain, and a PDZ domain, which are essential for vesicular localization and function. SNX27 interacts with proteins such as CYTIP and SR-4, facilitating the endocytic trafficking of various receptors, which is vital for maintaining cellular homeostasis and signaling. Four named isoforms of SNX27, resulting from alternative splicing, highlight the complexity and regulatory potential in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SNX27 Antibody (F-2) References:
- New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a receptor splice variant: roles in receptor targeting. | Joubert, L., et al. 2004. J Cell Sci. 117: 5367-79. PMID: 15466885
- Sorting nexins--unifying trends and new perspectives. | Carlton, J., et al. 2005. Traffic. 6: 75-82. PMID: 15634208
- The Phox (PX) domain proteins and membrane traffic. | Seet, LF. and Hong, W. 2006. Biochim Biophys Acta. 1761: 878-96. PMID: 16782399
- Proteomics identification of sorting nexin 27 as a diacylglycerol kinase zeta-associated protein: new diacylglycerol kinase roles in endocytic recycling. | Rincón, E., et al. 2007. Mol Cell Proteomics. 6: 1073-87. PMID: 17351151
- Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes. | MacNeil, AJ., et al. 2007. Biochem Biophys Res Commun. 359: 848-53. PMID: 17577583
- Polarization of endosomal SNX27 in migrating and tumor-engaged natural killer cells. | MacNeil, AJ. and Pohajdak, B. 2007. Biochem Biophys Res Commun. 361: 146-50. PMID: 17644068
- A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction. | Lunn, ML., et al. 2007. Nat Neurosci. 10: 1249-59. PMID: 17828261
- Subunit-specific regulation of Kir3 channels by sorting nexin 27. | Nassirpour, R. and Slesinger, PA. 2007. Channels (Austin). 1: 331-3. PMID: 18690037
- VPS26A-SNX27 Interaction-Dependent mGluR5 Recycling in Dorsal Horn Neurons Mediates Neuropathic Pain in Rats. | Lin, TB., et al. 2015. J Neurosci. 35: 14943-55. PMID: 26538661
- SNX27 Deletion Causes Hydrocephalus by Impairing Ependymal Cell Differentiation and Ciliogenesis. | Wang, X., et al. 2016. J Neurosci. 36: 12586-12597. PMID: 27974614
- SNX27-FERM-SNX1 complex structure rationalizes divergent trafficking pathways by SNX17 and SNX27. | Yong, X., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34462354
- SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling. | Simonetti, B., et al. 2022. PLoS Biol. 20: e3001601. PMID: 35417450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX27 Antibody (F-2) | sc-515707 | 200 µg/ml | $322.00 | |||
SNX27 Antibody (F-2) AC | sc-515707 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SNX27 Antibody (F-2) HRP | sc-515707 HRP | 200 µg/ml | $322.00 | |||
SNX27 Antibody (F-2) FITC | sc-515707 FITC | 200 µg/ml | $336.00 | |||
SNX27 Antibody (F-2) PE | sc-515707 PE | 200 µg/ml | $349.00 | |||
SNX27 Antibody (F-2) Alexa Fluor® 488 | sc-515707 AF488 | 200 µg/ml | $364.00 | |||
SNX27 Antibody (F-2) Alexa Fluor® 546 | sc-515707 AF546 | 200 µg/ml | $364.00 | |||
SNX27 Antibody (F-2) Alexa Fluor® 594 | sc-515707 AF594 | 200 µg/ml | $364.00 | |||
SNX27 Antibody (F-2) Alexa Fluor® 647 | sc-515707 AF647 | 200 µg/ml | $364.00 | |||
SNX27 Antibody (F-2) Alexa Fluor® 680 | sc-515707 AF680 | 200 µg/ml | $364.00 | |||
SNX27 Antibody (F-2) Alexa Fluor® 790 | sc-515707 AF790 | 200 µg/ml | $364.00 |