SNARK Antibody (E-8): sc-374283

SNARK Antibody (E-8) is a mouse monoclonal IgG2b antibody that detects SNARK in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SNARK, also known as NUAK2, is a 628 amino acid protein that plays a crucial role in cellular responses to stress, particularly in regulating cell tolerance to glucose starvation. SNARK contains a single protein kinase domain and is part of the serine/threonine protein kinase family, which is essential for the phosphorylation and dephosphorylation processes that govern various cellular functions, including cell division and apoptosis. SNARK′s ability to catalyze ATP-dependent phosphorylation using magnesium as a cofactor is vital for its function, as SNARK modulates the activity of target proteins involved in cellular stress responses. Furthermore, SNARK is implicated in promoting cell-cell detachment and may offer protection against Fap-1-mediated apoptosis, suggesting SNARK′s potential involvement in the motility and invasiveness of tumor cells. Anti-SNARK antibody (E-8) is an invaluable tool for researchers studying these critical pathways in cancer biology and cellular metabolism.

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SNARK Antibody (E-8) References:

1. NUAK2: an emerging acral melanoma oncogene.  |  Namiki, T., et al. 2011. Oncotarget. 2: 695-704. PMID: 21911917
2. NUAK2 Amplification Coupled with PTEN Deficiency Promotes Melanoma Development via CDK Activation.  |  Namiki, T., et al. 2015. Cancer Res. 75: 2708-15. PMID: 25832654
3. NUAK2 is a critical YAP target in liver cancer.  |  Yuan, WC., et al. 2018. Nat Commun. 9: 4834. PMID: 30446657
4. NUAK2 localization in normal skin and its expression in a variety of skin tumors with YAP.  |  Al-Busani, H., et al. 2020. J Dermatol Sci. 97: 143-151. PMID: 32001115
5. NUAK1 and NUAK2 Fine-Tune TGF-β Signaling.  |  van de Vis, RAJ., et al. 2021. Cancers (Basel). 13: PMID: 34282782
6. NUAK2 and RCan2 participate in the p53 mutant pro-tumorigenic network.  |  Mammarella, E., et al. 2021. Biol Direct. 16: 11. PMID: 34348766
7. NUAK2 silencing inhibits the proliferation, migration and epithelial‑to‑mesenchymal transition of cervical cancer cells via upregulating CYFIP2.  |  Li, Y., et al. 2021. Mol Med Rep. 24: PMID: 34558636
8. GPR65 promotes intestinal mucosal Th1 and Th17 cell differentiation and gut inflammation through downregulating NUAK2.  |  Lin, R., et al. 2022. Clin Transl Med. 12: e771. PMID: 35343079
9. Circ_0000033 up-regulates NUAK2 by sequestering miR-378a-3p to promote breast tumorigenesis.  |  Dai, Y., et al. 2023. Environ Mol Mutagen. 64: 359-370. PMID: 37357410
10. Enhanced SARS-CoV-2 entry via UPR-dependent AMPK-related kinase NUAK2.  |  Prasad, V., et al. 2023. Mol Cell. 83: 2559-2577.e8. PMID: 37421942
11. NUAK2 Inhibitors, KHKI-01128 and KHKI-01215, Exhibit Potent Anticancer Activity Against SW480 Colorectal Cancer Cells.  |  Lee, SH., et al. 2024. Anticancer Res. 44: 2909-2919. PMID: 38925848
12. NUAK2 mediated regulation of Schwann Cell proliferation and migration in peripheral nerve injury via YAP.  |  Zhang, W., et al. 2024. Heliyon. 10: e34127. PMID: 39071701

The preferred SNARK antibody is SNARK Antibody (C-12): sc-374348.