SNAP 25 Antibody (SP12): sc-20038
- SNAP 25 Antibody (SP12) is a mouse monoclonal IgG1 κ SNAP 25 antibody, cited in 32 publications, provided at 200 µg/ml
- raised against a crude synaptic preparation from the post mortem human brain
- SNAP 25 Antibody (SP12) is recommended for detection of SNAP 25 of broad species origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SNAP 25 Antibody (SP12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SNAP 25 Antibody (SP12) for WB and IHC(P) applications. These reagents are now offered in bundles with SNAP 25 Antibody (SP12) (see ordering information below).
SNAP 25 Antibody (SP12) is a mouse monoclonal IgG1 kappa light chain antibody that detects SNAP 25 protein of broad species origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-SNAP 25 antibody (SP12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SNAP 25 plays a crucial role in synaptic transmission as SNAP 25 is involved in the docking and fusion of synaptic vesicles with the presynaptic membrane, a process essential for neurotransmitter release. This function is vital for proper neuronal communication and overall brain function, as disruptions in SNAP 25 activity can lead to neurological disorders and impairments in synaptic plasticity. SNAP 25 interacts with several key proteins in the exocytosis pathway, including syntaxins and vesicle-associated membrane proteins (VAMPs), which together form a complex that facilitates the fusion of vesicles with the plasma membrane. Understanding SNAP 25 interactions and functions is important for elucidating the mechanisms underlying synaptic transmission and potential therapeutic targets for treating synaptic dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SNAP 25 Antibody (SP12) References:
- Stress-induced phosphorylation of SNAP-25. | Yamamori, S., et al. 2014. Neurosci Lett. 561: 182-7. PMID: 24374286
- SNAP-25, a Known Presynaptic Protein with Emerging Postsynaptic Functions. | Antonucci, F., et al. 2016. Front Synaptic Neurosci. 8: 7. PMID: 27047369
- A review of the role of synaptosomal-associated protein 25 (SNAP-25) in neurological disorders. | Noor, A. and Zahid, S. 2017. Int J Neurosci. 127: 805-811. PMID: 27734716
- Early Golgi Abnormalities and Neurodegeneration upon Loss of Presynaptic Proteins Munc18-1, Syntaxin-1, or SNAP-25. | Santos, TC., et al. 2017. J Neurosci. 37: 4525-4539. PMID: 28348137
- SNAP-25 gene family members differentially support secretory vesicle fusion. | Arora, S., et al. 2017. J Cell Sci. 130: 1877-1889. PMID: 28404788
- Botulinum neurotoxin C mutants reveal different effects of syntaxin or SNAP-25 proteolysis on neuromuscular transmission. | Zanetti, G., et al. 2017. PLoS Pathog. 13: e1006567. PMID: 28800600
- The Control of Neuronal Calcium Homeostasis by SNAP-25 and its Impact on Neurotransmitter Release. | Pozzi, D., et al. 2019. Neuroscience. 420: 72-78. PMID: 30476527
- miR-23a-3p and miR-181a-5p modulate SNAP-25 expression. | Agostini, S., et al. 2023. PLoS One. 18: e0279961. PMID: 36649268
- The Role of SNAP-25 in Autism Spectrum Disorders Onset Patterns. | Bolognesi, E., et al. 2023. Int J Mol Sci. 24: PMID: 37762342
- Serum NFL and tau, but not serum UCHL-1 and GFAP or CSF SNAP-25, NPTX2, or sTREM2, correlate with delirium in a 3-year retrospective analysis. | Piel, JHA., et al. 2024. Front Neurol. 15: 1356575. PMID: 38566855
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAP 25 Antibody (SP12) | sc-20038 | 200 µg/ml | $316.00 | |||
SNAP 25 Antibody (SP12): m-IgG Fc BP-HRP Bundle | sc-528353 | 200 µg Ab; 10 µg BP | $354.00 | |||
SNAP 25 Antibody (SP12): m-IgGκ BP-HRP Bundle | sc-520711 | 200 µg Ab, 40 µg BP | $354.00 | |||
SNAP 25 Antibody (SP12) AC | sc-20038 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SNAP 25 Antibody (SP12) HRP | sc-20038 HRP | 200 µg/ml | $316.00 | |||
SNAP 25 Antibody (SP12) FITC | sc-20038 FITC | 200 µg/ml | $330.00 | |||
SNAP 25 Antibody (SP12) PE | sc-20038 PE | 200 µg/ml | $343.00 | |||
SNAP 25 Antibody (SP12) Alexa Fluor® 488 | sc-20038 AF488 | 200 µg/ml | $357.00 | |||
SNAP 25 Antibody (SP12) Alexa Fluor® 546 | sc-20038 AF546 | 200 µg/ml | $357.00 | |||
SNAP 25 Antibody (SP12) Alexa Fluor® 594 | sc-20038 AF594 | 200 µg/ml | $357.00 | |||
SNAP 25 Antibody (SP12) Alexa Fluor® 647 | sc-20038 AF647 | 200 µg/ml | $357.00 | |||
SNAP 25 Antibody (SP12) Alexa Fluor® 680 | sc-20038 AF680 | 200 µg/ml | $357.00 | |||
SNAP 25 Antibody (SP12) Alexa Fluor® 790 | sc-20038 AF790 | 200 µg/ml | $357.00 |