SNAP 25 Antibody (H-1): sc-376713

SNAP 25 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SNAP 25 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-SNAP 25 antibody (H-1) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. SNAP 25 plays a crucial role in regulating neurotransmitter release, functioning as a key component of the SNARE complex, which is essential for synaptic vesicle fusion with the presynaptic membrane. This process enables neurotransmitter release into the synaptic cleft, facilitating communication between neurons. Proper functioning of SNAP 25 is critical for normal neuronal activity, and dysregulation has been implicated in various neurological disorders, including schizophrenia and autism spectrum disorders. SNAP 25 also interacts with other proteins involved in exocytosis, such as syntaxins and VAMPs, forming a complex necessary for effective neurotransmitter release. Understanding SNAP 25 and its interactions is essential for elucidating synaptic transmission mechanisms and potential therapeutic targets for neurological diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SNAP 25 Antibody (H-1) References:

1. Neuronal differentiation is accompanied by increased levels of SNAP-25 protein in fetal rat primary cortical neurons: implications in neuronal plasticity and Alzheimer's disease.  |  Bailey, JA. and Lahiri, DK. 2006. Ann N Y Acad Sci. 1086: 54-65. PMID: 17185505
2. Cleavage of SNAP-25 ameliorates cancer pain in a mouse model of melanoma.  |  Olbrich, K., et al. 2017. Eur J Pain. 21: 101-111. PMID: 27301493
3. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters.  |  Toft-Bertelsen, TL., et al. 2016. Mol Biol Cell. 27: 3329-3341. PMID: 27605709
4. SNAP-25 Polymorphisms in Autism Spectrum Disorder: A Pilot Study towards a Possible Endophenotype.  |  Mensi, MM., et al. 2023. Pediatr Rep. 15: 766-773. PMID: 38133436
5. The syntaxin family of vesicular transport receptors.  |  Bennett, MK., et al. 1993. Cell. 74: 863-73. PMID: 7690687
6. Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine.  |  Edelmann, L., et al. 1995. EMBO J. 14: 224-31. PMID: 7835333
7. Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site.  |  McMahon, HT. and Südhof, TC. 1995. J Biol Chem. 270: 2213-7. PMID: 7836452
8. Exocytosis relating proteins in the nervous system.  |  Yamaguchi, K. and Akagawa, K. 1994. Neurosci Res. 20: 289-92. PMID: 7870381
9. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly.  |  Hayashi, T., et al. 1994. EMBO J. 13: 5051-61. PMID: 7957071
10. A role for synaptotagmin (p65) in regulated exocytosis.  |  Elferink, LA., et al. 1993. Cell. 72: 153-9. PMID: 8422678
11. Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis.  |  Barnard, RJ., et al. 1997. J Cell Biol. 139: 875-83. PMID: 9362506
12. Structural organization of the synaptic exocytosis core complex.  |  Lin, RC. and Scheller, RH. 1997. Neuron. 19: 1087-94. PMID: 9390521

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SNAP 25 Antibody (H-1) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-SNAP 25 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SNAP 25 Antibody (H-1) Specifications

Alternate Product Name: SNAP 25

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 91-140

Epitope Target: SNAP 25

Epitope Species: human

Product Reactivity/Detection: SNAP 25

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for SNAP 25 Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin