SNAP 25 Antibody (SP12): sc-20038

SNAP 25 Antibody (SP12) is a mouse monoclonal IgG1 kappa light chain antibody that detects SNAP 25 protein of broad species origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-SNAP 25 antibody (SP12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SNAP 25 plays a crucial role in synaptic transmission as SNAP 25 is involved in the docking and fusion of synaptic vesicles with the presynaptic membrane, a process essential for neurotransmitter release. This function is vital for proper neuronal communication and overall brain function, as disruptions in SNAP 25 activity can lead to neurological disorders and impairments in synaptic plasticity. SNAP 25 interacts with several key proteins in the exocytosis pathway, including syntaxins and vesicle-associated membrane proteins (VAMPs), which together form a complex that facilitates the fusion of vesicles with the plasma membrane. Understanding SNAP 25 interactions and functions is important for elucidating the mechanisms underlying synaptic transmission and potential therapeutic targets for treating synaptic dysfunction.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SNAP 25 Antibody (SP12) References:

1. Stress-induced phosphorylation of SNAP-25.  |  Yamamori, S., et al. 2014. Neurosci Lett. 561: 182-7. PMID: 24374286
2. SNAP-25, a Known Presynaptic Protein with Emerging Postsynaptic Functions.  |  Antonucci, F., et al. 2016. Front Synaptic Neurosci. 8: 7. PMID: 27047369
3. A review of the role of synaptosomal-associated protein 25 (SNAP-25) in neurological disorders.  |  Noor, A. and Zahid, S. 2017. Int J Neurosci. 127: 805-811. PMID: 27734716
4. Early Golgi Abnormalities and Neurodegeneration upon Loss of Presynaptic Proteins Munc18-1, Syntaxin-1, or SNAP-25.  |  Santos, TC., et al. 2017. J Neurosci. 37: 4525-4539. PMID: 28348137
5. SNAP-25 gene family members differentially support secretory vesicle fusion.  |  Arora, S., et al. 2017. J Cell Sci. 130: 1877-1889. PMID: 28404788
6. Botulinum neurotoxin C mutants reveal different effects of syntaxin or SNAP-25 proteolysis on neuromuscular transmission.  |  Zanetti, G., et al. 2017. PLoS Pathog. 13: e1006567. PMID: 28800600
7. The Control of Neuronal Calcium Homeostasis by SNAP-25 and its Impact on Neurotransmitter Release.  |  Pozzi, D., et al. 2019. Neuroscience. 420: 72-78. PMID: 30476527
8. miR-23a-3p and miR-181a-5p modulate SNAP-25 expression.  |  Agostini, S., et al. 2023. PLoS One. 18: e0279961. PMID: 36649268
9. The Role of SNAP-25 in Autism Spectrum Disorders Onset Patterns.  |  Bolognesi, E., et al. 2023. Int J Mol Sci. 24: PMID: 37762342
10. Serum NFL and tau, but not serum UCHL-1 and GFAP or CSF SNAP-25, NPTX2, or sTREM2, correlate with delirium in a 3-year retrospective analysis.  |  Piel, JHA., et al. 2024. Front Neurol. 15: 1356575. PMID: 38566855

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SNAP 25 Antibody (SP12) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-SNAP 25 Antibody (SP12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SNAP 25 Antibody (SP12) Specifications

Alternate Product Name: Synaptosome Associated Protein 25

Product Clone Name: SP12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SNAP 25

Epitope Species: human

Product Reactivity/Detection: SNAP 25

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for SNAP 25 Antibody (SP12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SNAP 25 antibody is SNAP 25 Antibody (H-1): sc-376713.