Smurf2 Antibody (C-5): sc-518164
- Smurf2 Antibody (C-5) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 204-253 of Smurf2 of human origin
- recommended for detection of Smurf2 of human origin by WB, IP, IF and ELISA
- Anti-Smurf2 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Smurf2 Antibody (C-5) for WB applications. These reagents are now offered in bundles with Smurf2 Antibody (C-5) (see ordering information below).
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Smurf2 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Smurf2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Smurf2 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smurf2, a member of the Hect family of proteins, plays a crucial role in the ubiquitin-proteasome pathway, specifically functioning as an E3 ubiquitin ligase. Smurf2 is essential for regulating the degradation of receptor-regulated SMADs, which are pivotal in the transforming growth factor-beta (TGF-β) signaling pathway. By selectively interacting with R-Smads such as Smad1, Smad2, and Smad3, Smurf2 facilitates their ubiquitination and subsequent degradation, thereby modulating cellular responses to TGF-β signals. Nuclear localization of Smurf2 allows for SMAD activity regulation; however, Smurf2 interaction with Smad7 leads to nuclear export and recruitment to the activated TGF-β receptor, promoting Smad7 degradation. This dynamic regulation underscores Smurf2′s importance in maintaining cellular homeostasis and potential implications in various diseases, including cancer and fibrosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Smurf2 Antibody (C-5) References:
- A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. | Zhu, H., et al. 1999. Nature. 400: 687-93. PMID: 10458166
- Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling. | Lin, X., et al. 2000. J Biol Chem. 275: 36818-22. PMID: 11016919
- Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation. | Kavsak, P., et al. 2000. Mol Cell. 6: 1365-75. PMID: 11163210
- The Post-translational Modifications of Smurf2 in TGF-β Signaling. | Bai, Y. and Ying, Y. 2020. Front Mol Biosci. 7: 128. PMID: 32733916
- Flavin Containing Monooxygenase 2 Prevents Cardiac Fibrosis via CYP2J3-SMURF2 Axis. | Ni, C., et al. 2022. Circ Res. 101161CIRCRESAHA122320538. PMID: 35861735
- SMURF2 facilitates ubiquitin-mediated degradation of ID2 to attenuate lung cancer cell proliferation. | Han, M., et al. 2023. Int J Biol Sci. 19: 3324-3340. PMID: 37497010
- Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression. | Pi, Y., et al. 2023. Cell Death Differ. 30: 2382-2392. PMID: 37828084
- SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation. | Zhang, W., et al. 2023. Mol Cell. 83: 4352-4369.e8. PMID: 38016474
- FOXO1 regulates RUNX2 ubiquitination through SMURF2 in calcific aortic valve disease. | Jiang, C., et al. 2024. Redox Biol. 73: 103215. PMID: 38810422
- A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. | Huibregtse, JM., et al. 1995. Proc Natl Acad Sci U S A. 92: 2563-7. PMID: 7708685
- The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. | Scheffner, M., et al. 1993. Cell. 75: 495-505. PMID: 8221889
- The ubiquitin system. | Hershko, A. and Ciechanover, A. 1998. Annu Rev Biochem. 67: 425-79. PMID: 9759494
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smurf2 Antibody (C-5) | sc-518164 | 200 µg/ml | $322.00 | |||
Smurf2 Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-550676 | 200 µg Ab; 10 µg BP | $361.00 | |||
Smurf2 Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-550671 | 200 µg Ab; 40 µg BP | $361.00 | |||
Smurf2 Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-551787 | 200 µg Ab; 20 µg BP | $361.00 | |||
Smurf2 Antibody (C-5) AC | sc-518164 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Smurf2 Antibody (C-5) HRP | sc-518164 HRP | 200 µg/ml | $322.00 | |||
Smurf2 Antibody (C-5) FITC | sc-518164 FITC | 200 µg/ml | $336.00 | |||
Smurf2 Antibody (C-5) PE | sc-518164 PE | 200 µg/ml | $349.00 | |||
Smurf2 Antibody (C-5) Alexa Fluor® 488 | sc-518164 AF488 | 200 µg/ml | $364.00 | |||
Smurf2 Antibody (C-5) Alexa Fluor® 546 | sc-518164 AF546 | 200 µg/ml | $364.00 | |||
Smurf2 Antibody (C-5) Alexa Fluor® 594 | sc-518164 AF594 | 200 µg/ml | $364.00 | |||
Smurf2 Antibody (C-5) Alexa Fluor® 647 | sc-518164 AF647 | 200 µg/ml | $364.00 | |||
Smurf2 Antibody (C-5) Alexa Fluor® 680 | sc-518164 AF680 | 200 µg/ml | $364.00 | |||
Smurf2 Antibody (C-5) Alexa Fluor® 790 | sc-518164 AF790 | 200 µg/ml | $364.00 |