Smurf1 Antibody (45-K): sc-100616
- Smurf1 Antibody (45-K) is a mouse monoclonal IgG2a κ Smurf1 antibody, cited in 37 publications, provided at 100 µg/ml
- raised against an internal portion of Smurf1 of human origin
- recommended for detection of Smurf1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Smurf1 Antibody (45-K) for WB and IHC(P) applications. These reagents are now offered in bundles with Smurf1 Antibody (45-K) (see ordering information below).
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Smurf1 Antibody (45-K) is a mouse monoclonal IgG2a kappa light chain antibody that detects Smurf1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Smurf1 antibody (45-K) is available as the non-conjugated format. Smurf1, also known as SMAD Specific E3 Ubiquitin Protein Ligase 1, plays a crucial role in regulating the bone morphogenetic protein (BMP) signaling pathway by mediating ubiquitination and subsequent degradation of receptor-regulated SMADs. This function maintains cellular homeostasis and ensures proper signaling, as dysregulation of SMAD proteins can lead to various pathological conditions, including cancer and fibrosis. Smurf1 selectively interacts with specific SMAD proteins, facilitating ubiquitination, which marks them for degradation by the 26S proteasome. This targeted degradation fine-tunes BMP signaling, allowing cells to respond appropriately to extracellular cues. Smurf1′s interaction with other proteins in the ubiquitin-conjugating pathway highlights its significance in cellular signaling and protein turnover, making Smurf1 a key focus for research in developmental biology and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Smurf1 Antibody (45-K) References:
- A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. | Zhu, H., et al. 1999. Nature. 400: 687-93. PMID: 10458166
- Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling. | Lin, X., et al. 2000. J Biol Chem. 275: 36818-22. PMID: 11016919
- Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation. | Kavsak, P., et al. 2000. Mol Cell. 6: 1365-75. PMID: 11163210
- A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. | Huibregtse, JM., et al. 1995. Proc Natl Acad Sci U S A. 92: 2563-7. PMID: 7708685
- The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53. | Scheffner, M., et al. 1993. Cell. 75: 495-505. PMID: 8221889
- The ubiquitin system. | Hershko, A. and Ciechanover, A. 1998. Annu Rev Biochem. 67: 425-79. PMID: 9759494
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smurf1 Antibody (45-K) | sc-100616 | 100 µg/ml | $333.00 | |||
Smurf1 Antibody (45-K): m-IgG Fc BP-HRP Bundle | sc-537279 | 100 µg Ab; 10 µg BP | $354.00 | |||
Smurf1 Antibody (45-K): m-IgGκ BP-HRP Bundle | sc-534510 | 100 µg Ab; 40 µg BP | $354.00 | |||
Smurf1 Antibody (45-K): m-IgG2a BP-HRP Bundle | sc-547957 | 100 µg Ab; 10 µg BP | $354.00 |