Smurf1 Antibody (C-11): sc-518118
- Smurf1 Antibody (C-11) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 289-308 of Smurf1 of human origin
- recommended for detection of Smurf1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Smurf1 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Smurf1 Antibody (C-11) for WB applications. This reagent is now offered in a bundle with Smurf1 Antibody (C-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Smurf1 Antibody (C-11) is a mouse monoclonal IgG3 kappa light chain antibody that detects Smurf1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Smurf1 antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Smurf1, a member of the Hect family of proteins, plays a crucial role in the ubiquitin-proteasome pathway by functioning as an E3 ubiquitin ligase. Smurf1 (C-11) monoclonal antibody enables research into how Smurf1 regulates the degradation of receptor-regulated SMADs, particularly those involved in the bone morphogenetic protein (BMP) signaling pathway. By selectively catalyzing the transfer of ubiquitin to these SMADs, Smurf1 facilitates their recognition and subsequent degradation by the 26S proteasome, thereby modulating cellular responses to BMP signals. The precise regulation of SMAD levels is vital for maintaining proper signaling and cellular functions, as dysregulation can lead to various pathologies, including cancer and developmental disorders. With specific interaction with SMAD proteins, Smurf1 serves as a key player in the intricate network of cellular signaling, making Smurf1 (C-11) antibody an invaluable tool for researchers studying these critical pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Smurf1 Antibody (C-11) References:
- Ubiquitination of UVRAG by SMURF1 promotes autophagosome maturation and inhibits hepatocellular carcinoma growth. | Feng, X., et al. 2019. Autophagy. 15: 1130-1149. PMID: 30686098
- Deletion of Smurf1 attenuates liver steatosis via stabilization of p53. | Lin, W., et al. 2022. Lab Invest. 102: 1075-1087. PMID: 35672379
- SMURF1 attenuates endoplasmic reticulum stress by promoting the degradation of KEAP1 to activate NRF2 antioxidant pathway. | Dong, L., et al. 2023. Cell Death Dis. 14: 361. PMID: 37316499
- Smurf1: A possible therapeutic target in dry age-related macular degeneration. | Li, D., et al. 2023. Exp Eye Res. 233: 109549. PMID: 37348673
- Selective Smurf1 E3 ligase inhibitors that prevent transthiolation. | Chuong, P. and Statsyuk, A. 2023. bioRxiv.. PMID: 37873387
- SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis. | Xia, Q., et al. 2024. Autophagy. 20: 735-751. PMID: 37909662
- SMURF1 activates the cGAS/STING/IFN-1 signal axis by mediating YY1 ubiquitination to accelerate the progression of lupus nephritis. | Li, X., et al. 2023. Autoimmunity. 56: 2281235. PMID: 37994046
- Smurf1 Modulates Smad Signaling Pathway in Fibrotic Cataract Formation. | Jiang, F., et al. 2024. Invest Ophthalmol Vis Sci. 65: 18. PMID: 38324299
- Targeting Smurf1 to block PDK1-Akt signaling in KRAS-mutated colorectal cancer. | Peng, Z., et al. 2025. Nat Chem Biol. 21: 59-70. PMID: 39039255
- SMURF1 mediates damaged lysosomal homeostasis by ubiquitinating PPP3CB to promote the activation of TFEB. | Xia, Q., et al. 2024. Autophagy. 1-18. PMID: 39324484
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smurf1 Antibody (C-11) | sc-518118 | 200 µg/ml | $322.00 | |||
Smurf1 Antibody (C-11): m-IgGκ BP-HRP Bundle | sc-525383 | 200 µg Ab, 40 µg BP | $361.00 | |||
Smurf1 Antibody (C-11) AC | sc-518118 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Smurf1 Antibody (C-11) HRP | sc-518118 HRP | 200 µg/ml | $322.00 | |||
Smurf1 Antibody (C-11) FITC | sc-518118 FITC | 200 µg/ml | $336.00 | |||
Smurf1 Antibody (C-11) PE | sc-518118 PE | 200 µg/ml | $349.00 | |||
Smurf1 Antibody (C-11) Alexa Fluor® 488 | sc-518118 AF488 | 200 µg/ml | $364.00 | |||
Smurf1 Antibody (C-11) Alexa Fluor® 546 | sc-518118 AF546 | 200 µg/ml | $364.00 | |||
Smurf1 Antibody (C-11) Alexa Fluor® 594 | sc-518118 AF594 | 200 µg/ml | $364.00 | |||
Smurf1 Antibody (C-11) Alexa Fluor® 647 | sc-518118 AF647 | 200 µg/ml | $364.00 | |||
Smurf1 Antibody (C-11) Alexa Fluor® 680 | sc-518118 AF680 | 200 µg/ml | $364.00 | |||
Smurf1 Antibody (C-11) Alexa Fluor® 790 | sc-518118 AF790 | 200 µg/ml | $364.00 |