Date published: 2025-9-10

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sMtCK Antibody (C-6): sc-390489

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Datasheets
  • sMtCK Antibody (C-6) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 1-95 mapping at the N-terminus of sMtCK of human origin
  • Anti-sMtCK Antibody (C-6) is recommended for detection of sMtCK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-sMtCK Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of sMtCK (C-6): sc-390489.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for sMtCK Antibody (C-6) for WB and IHC(P) applications. These reagents are now offered in bundles with sMtCK Antibody (C-6) (see ordering information below).

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    sMtCK Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects sMtCK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-sMtCK antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. sMtCK, a member of the creatine kinase family, plays a crucial role in cellular energy metabolism by catalyzing the reversible transfer of phosphate from creatine to adenosine diphosphate (ADP) to form adenosine triphosphate (ATP), particularly in tissues with high energy demands such as muscle and brain. This enzymatic activity maintains ATP levels during periods of fluctuating energy requirements, ensuring cellular processes, including muscle contraction and neurotransmission, function optimally. Proper regulation of sMtCK is essential for cellular health, as dysregulation can lead to energy deficits and contribute to various pathologies, including muscular and neurological disorders. By providing localized ATP, sMtCK regulates calcium pumps and maintains calcium homeostasis, highlighting its importance in cellular function and overall metabolic health.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    sMtCK Antibody (C-6)

    sc-390489
    200 µg/ml
    $316.00

    sMtCK Antibody (C-6): m-IgG Fc BP-HRP Bundle

    sc-530170
    200 µg Ab; 10 µg BP
    $354.00

    sMtCK Antibody (C-6): m-IgGκ BP-HRP Bundle

    sc-523557
    200 µg Ab, 40 µg BP
    $354.00

    sMtCK Antibody (C-6): m-IgG1 BP-HRP Bundle

    sc-543906
    200 µg Ab; 20 µg BP
    $354.00

    sMtCK Antibody (C-6) AC

    sc-390489 AC
    500 µg/ml, 25% agarose
    $416.00

    sMtCK Antibody (C-6) HRP

    sc-390489 HRP
    200 µg/ml
    $316.00

    sMtCK Antibody (C-6) FITC

    sc-390489 FITC
    200 µg/ml
    $330.00

    sMtCK Antibody (C-6) PE

    sc-390489 PE
    200 µg/ml
    $343.00

    sMtCK Antibody (C-6) Alexa Fluor® 488

    sc-390489 AF488
    200 µg/ml
    $357.00

    sMtCK Antibody (C-6) Alexa Fluor® 546

    sc-390489 AF546
    200 µg/ml
    $357.00

    sMtCK Antibody (C-6) Alexa Fluor® 594

    sc-390489 AF594
    200 µg/ml
    $357.00

    sMtCK Antibody (C-6) Alexa Fluor® 647

    sc-390489 AF647
    200 µg/ml
    $357.00

    sMtCK Antibody (C-6) Alexa Fluor® 680

    sc-390489 AF680
    200 µg/ml
    $357.00

    sMtCK Antibody (C-6) Alexa Fluor® 790

    sc-390489 AF790
    200 µg/ml
    $357.00

    Can sMtCK (C-6): sc-390489 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Cweed
    Thank you for your question. Yes, sMtCK (C-6): sc-390489 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
    Date published: 2015-03-19
    Rated 5 out of 5 by from Nice Western Blot data of sMtCK expressionNice Western Blot data of sMtCK expression in rat skeletal muscle and human skeletal muscle tissue extracts. -SCBT QC
    Date published: 2013-02-04
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    sMtCK Antibody (C-6) is rated 5.0 out of 5 by 2.
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