SMP30 Antibody (H-4): sc-377184

SMP30 Antibody (H-4) is a mouse monoclonal IgG2b antibody that detects senescence marker protein-30 (SMP30) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMP30 plays a crucial role in maintaining intracellular calcium homeostasis by modulating the activity of the plasma membrane calcium pump, which is vital for cellular function and survival. By regulating calcium levels, SMP30 helps protect cells from apoptosis, thereby contributing to tissue health and longevity. SMP30 is predominantly expressed in the liver, kidney, and submandibular gland, with localization in the renal proximal tubular epithelium and hepatocytes being particularly significant for protective functions. Notably, SMP30 expression increases during tissue maturation and decreases with aging in an androgen-independent manner, highlighting a potential role in age-related cellular processes. The promoter sequence of the mouse SMP30 gene contains binding sites for various transcription factors, including Sp1, AP2, CCAAT box, Lyf-1, and GATA-1, which may influence expression and activity in different physiological contexts. Anti-SMP30 antibody (H-4) is an essential tool for researchers studying the implications of SMP30 in cellular aging and calcium regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SMP30 Antibody (H-4) References:

1. SMP30 deficiency causes increased oxidative stress in brain.  |  Son, TG., et al. 2006. Mech Ageing Dev. 127: 451-7. PMID: 16500693
2. Soluble expression of recombinant human SMP30 for detecting serum SMP30 antibody levels in hepatocellular carcinoma patients.  |  Zhang, SC., et al. 2013. Asian Pac J Cancer Prev. 14: 2383-6. PMID: 23725145
3. Lepr(db/db) Mice with senescence marker protein-30 knockout (Lepr(db/db)Smp30(Y/-)) exhibit increases in small dense-LDL and severe fatty liver despite being fed a standard diet.  |  Kondo, Y., et al. 2013. PLoS One. 8: e65698. PMID: 23755269
4. Stem Cell Replacement Improves Expression of SMP30 in db/db Mice.  |  Li, M., et al. 2015. Int J Mol Sci. 16: 29971-9. PMID: 26694363
5. Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats.  |  Lin, PH., et al. 2016. Sci Rep. 6: 32085. PMID: 27553527
6. Senescence marker protein 30 confers neuroprotection in oxygen-glucose deprivation/reoxygenation-injured neurons through modulation of Keap1/Nrf2 signaling: Role of SMP30 in OGD/R-induced neuronal injury.  |  Liu, W., et al. 2021. Hum Exp Toxicol. 40: 472-482. PMID: 32909858
7. Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals.  |  Baek, SM., et al. 2021. Int J Mol Sci. 22: PMID: 33652881
8. Release of SMP30 in Extracellular Vesicles under Conditions of Ascorbic Acid Deficiency Is Involved with Acute Phase Response in ODS Rat.  |  Arakawa, K., et al. 2023. J Nutr Sci Vitaminol (Tokyo). 69: 420-427. PMID: 38171814
9. Gene regulation of senescence marker protein-30 (SMP30): coordinated up-regulation with tissue maturation and gradual down-regulation with aging.  |  Fujita, T., et al. 1996. Mech Ageing Dev. 87: 219-29. PMID: 8794449
10. Senescence marker protein-30 (SMP30) rescues cell death by enhancing plasma membrane Ca(2+)-pumping activity in Hep G2 cells.  |  Fujita, T., et al. 1998. Biochem Biophys Res Commun. 250: 374-80. PMID: 9753637

The preferred SMP30 antibody is SMP30 Antibody (G-10): sc-374019.