SMP30 Antibody (E-11): sc-390098

SMP30 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMP30 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SMP30 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Senescence marker protein-30 (SMP30) plays a crucial role in maintaining intracellular calcium homeostasis by modulating plasma membrane calcium pump activity, which is vital for cellular function and survival. This regulation of calcium levels helps protect cells from apoptosis, thereby influencing tissue health and longevity. SMP30 is predominantly expressed in liver, kidney, and submandibular gland, with localization in hepatocytes and renal proximal tubular epithelium in kidney. Notably, SMP30 expression increases during tissue maturation and decreases with aging in an androgen-independent manner, highlighting SMP30′s potential role in age-related cellular processes. The promoter sequence for mouse SMP30 gene contains binding sites for various transcription factors, including Sp1, AP2, CCAAT box, Lyf-1, and GATA-1, indicating a complex regulatory mechanism that governs SMP30 expression.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SMP30 Antibody (E-11) References:

1. SMP30 deficiency in mice causes an accumulation of neutral lipids and phospholipids in the liver and shortens the life span.  |  Ishigami, A., et al. 2004. Biochem Biophys Res Commun. 315: 575-80. PMID: 14975739
2. Purification of senescence marker protein-30 (SMP30) and its androgen-independent decrease with age in the rat liver.  |  Fujita, T., et al. 1992. Biochim Biophys Acta. 1116: 122-8. PMID: 1581340
3. The redox-sensitive DNA binding sites responsible for age-related downregulation of SMP30 by ERK pathway and reversal by calorie restriction.  |  Jung, KJ., et al. 2006. Antioxid Redox Signal. 8: 671-80. PMID: 16677110
4. Accelerated tubular cell senescence in SMP30 knockout mice.  |  Yumura, W., et al. 2006. Histol Histopathol. 21: 1151-6. PMID: 16874657
5. Establishment and characterization of hepatocytes from an Immortomouse/SMP30/GNL knockout mouse hybrid lacking vitamin C to study vitamin C transport.  |  Amano, A., et al. 2011. J Biochem. 150: 671-8. PMID: 21903636
6. Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice.  |  Ishikawa, Y., et al. 2012. Exp Eye Res. 94: 85-9. PMID: 22155581
7. Ascorbic acid deficiency leads to epidermal atrophy and UVB-induced skin pigmentation in SMP30/GNL knockout hairless mice.  |  Sato, Y., et al. 2012. J Invest Dermatol. 132: 2112-5. PMID: 22495180
8. Senescence marker protein-30 (SMP30) deficiency impairs myocardium-induced dilation of coronary arterioles associated with reactive oxygen species.  |  Mizukami, H., et al. 2013. Int J Mol Sci. 14: 9408-23. PMID: 23629672
9. Comparative analysis of the metal-dependent structural and functional properties of mouse and human SMP30.  |  Dutta, RK., et al. 2019. PLoS One. 14: e0218629. PMID: 31220150
10. Gene regulation of senescence marker protein-30 (SMP30): coordinated up-regulation with tissue maturation and gradual down-regulation with aging.  |  Fujita, T., et al. 1996. Mech Ageing Dev. 87: 219-29. PMID: 8794449

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMP30 Antibody (E-11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-SMP30 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMP30 Antibody (E-11) Specifications

Alternate Product Name: SMP30

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-299

Epitope Target: SMP30

Epitope Species: human

Product Reactivity/Detection: SMP30

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for SMP30 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SMP30 antibody is SMP30 Antibody (G-10): sc-374019.