Anti-SMN Antibody (2B1) recommended for detection of SMN of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)

Anti-SMN Antibody (2B1): sc-32313

Anti-SMN Antibody (2B1) is rated 4.7 out of 5 by 13.
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Datasheets

    • Anti-SMN Antibody (2B1) is a mouse monoclonal IgG1 κ SMN antibody, cited in 17 publications, provided at 200 µg/ml
    • raised against purified human recombinant His6 tagged-SMN protein
    • SMN Antibody (2B1) is recommended for detection of SMN of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)
    • Anti-SMN Antibody (2B1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SMN (2B1): sc-32313.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SMN Antibody (2B1) for WB and IHC(P) applications. These reagents are now offered in bundles with SMN Antibody (2B1) (see ordering information below).
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Hi there! I would love to receive samples from the SMN Antibody (2B1) and SMN Antibody (2B1)-HRP to test. Would that be possible? Many thanks, Flavia

Asked by: Flavia Nery
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

Can SMN (2B1): sc-32313 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SMN (2B1): sc-32313 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great antibody 申请的试用装,50uL体积,做WB的稀释度是1:800,A549细胞,条带很干净。非常感谢Santa的试用装申请活动
Date published: 2019-12-09
Rated 5 out of 5 by from HCT116細胞の免疫染色できました。 サンプルをいただいて、HCT116細胞の免疫染色を行いましたが、問題なく染色できました。ウェスタンブロットも少し非特異的なバンドが見えましたが、クリティカルではありませんでした。
Date published: 2019-04-17
Rated 5 out of 5 by from an excellent antibody for both WB and IF This antibody works well for WB (1:500 dilution, 40 ug U251 Lysate) and IF (1:200 dililution, formaldehyde-fixed HeLa cells)
Date published: 2017-07-05
Rated 1 out of 5 by from not so great enough the antibody does not work in leukemia cells, especially in IF
Date published: 2017-03-07
Rated 5 out of 5 by from Specific and Versatile This antibody shows great signal and specific staining with no background issues in WB, IF and IHC!
Date published: 2017-01-24
Rated 5 out of 5 by from Published for CoIP in human HeLa cells Published for CoIP in human HeLa cells. -SCBT Publication Review
Date published: 2015-07-07
Rated 5 out of 5 by from Good publishable data in IP Good publishable data in IP, WB and IF in human U20S cells. -SCBT Publication Review
Date published: 2015-06-13
Rated 5 out of 5 by from Strong band in WB in 293 Strong band in WB in 293, HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-05-07
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