SMN Antibody (2B1): sc-32313

SMN Antibody (2B1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the SMN protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). SMN (2B1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The survival motor neuron (SMN) protein plays a critical role in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), which are essential for pre-mRNA splicing, a fundamental process in gene expression regulation. The SMN complex, which includes several Gemin proteins, is vital for the biogenesis of snRNPs in the cytoplasm and their subsequent recycling in the nucleus. This complex is predominantly located in the cytoplasm and the nucleus, where SMN is concentrated in subnuclear structures known as gems. Proper functioning of SMN is crucial, as mutations or deletions in the SMN gene lead to spinal muscular atrophy (SMA), a severe neurodegenerative disorder characterized by the loss of motor neurons in the spinal cord. SMN is expressed in various tissues, including the brain, kidney, liver, spinal cord, and to a lesser extent in skeletal and cardiac muscle, highlighting SMN′s importance in maintaining neuronal health and function.

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SMN Antibody (2B1) References:

1. A single nucleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy.  |  Lorson, CL., et al. 1999. Proc Natl Acad Sci U S A. 96: 6307-11. PMID: 10339583
2. A single nucleotide difference that alters splicing patterns distinguishes the SMA gene SMN1 from the copy gene SMN2.  |  Monani, UR., et al. 1999. Hum Mol Genet. 8: 1177-83. PMID: 10369862
3. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.  |  Meister, G., et al. 2000. Hum Mol Genet. 9: 1977-86. PMID: 10942426
4. SMN interacts with a novel family of hnRNP and spliceosomal proteins.  |  Mourelatos, Z., et al. 2001. EMBO J. 20: 5443-52. PMID: 11574476
5. Risdiplam distributes and increases SMN protein in both the central nervous system and peripheral organs.  |  Poirier, A., et al. 2018. Pharmacol Res Perspect. 6: e00447. PMID: 30519476
6. Biodistribution of onasemnogene abeparvovec DNA, mRNA and SMN protein in human tissue.  |  Thomsen, G., et al. 2021. Nat Med. 27: 1701-1711. PMID: 34608334
7. SMN regulates GEMIN5 expression and acts as a modifier of GEMIN5-mediated neurodegeneration.  |  Fortuna, TR., et al. 2023. Acta Neuropathol. 146: 477-498. PMID: 37369805
8. The SMN complex drives structural changes in human snRNAs to enable snRNP assembly.  |  Pánek, J., et al. 2023. Nat Commun. 14: 6580. PMID: 37852981
9. SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy.  |  Valsecchi, V., et al. 2023. Commun Biol. 6: 1155. PMID: 37957344
10. Nucleolar reorganization after cellular stress is orchestrated by SMN shuttling between nuclear compartments.  |  Musawi, S., et al. 2023. Nat Commun. 14: 7384. PMID: 37968267
11. The survival motor neuron protein in spinal muscular atrophy.  |  Coovert, DD., et al. 1997. Hum Mol Genet. 6: 1205-14. PMID: 9259265
12. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis.  |  Fischer, U., et al. 1997. Cell. 90: 1023-9. PMID: 9323130

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMN Antibody (2B1) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-SMN Antibody (2B1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMN Antibody (2B1) Specifications

Alternate Product Name: Gemin 1

Product Clone Name: 2B1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SMN

Epitope Species: human

Product Reactivity/Detection: SMN

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for SMN Antibody (2B1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin