SmcX Antibody (F-12): sc-373935

SmcX Antibody (F-12) is a mouse monoclonal IgG2a antibody that detects SmcX in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SmcX, also known as JARID1C, MRXJ, KDM5C, or XE169, is a crucial nuclear protein that plays a significant role in the regulation of gene expression and chromatin dynamics. SmcX contains one ARID domain, one JmjC domain, one JmjN domain, and two PHD-type zinc fingers, classifying SmcX within the JARID1 histone demethylase family. SmcX is predominantly expressed in the brain and skeletal muscle, where SmcX functions as a histone demethylase, specifically removing methyl groups from lysine residues on Histone H3. This demethylation is vital for maintaining the histone code, which influences transcriptional regulation and chromatin remodeling, thereby impacting cellular processes such as differentiation and development. SmcX′s ability to bind iron and α-ketoglutarate as cofactors enhances enzymatic activity, and SmcX can also recruit histone deacetylases to neuron silencer elements, effectively repressing the transcription of neuronal genes. Importantly, defects in the gene encoding SmcX are linked to X-linked mental retardation (XLMR), a condition that results in cognitive impairment and low IQ, highlighting SmcX′s critical role in neurodevelopment. Additionally, multiple isoforms of SmcX arise from alternative splicing, which may contribute to functional diversity in various cellular contexts.

The preferred SmcX antibody is SmcX Antibody (G-10): sc-376255.