SmcX Antibody (G-10): sc-376255

SmcX Antibody (G-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects SmcX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SmcX (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SmcX protein, also known as JARID1C (jumonji, AT rich interactive domain 1C), MRXJ, KDM5C, or XE169, is a crucial nuclear protein that plays a significant role in epigenetic regulation. SmcX contains one ARID domain, one JmjC domain, one JmjN domain, and two PHD-type zinc fingers, classifying SmcX within the JARID1 histone demethylase family. SmcX is predominantly expressed in brain and skeletal muscle, where SmcX functions as a histone demethylase, specifically removing methyl groups from lysine residues on Histone H3. This activity is vital for maintaining the histone code, which influences transcriptional regulation and chromatin remodeling, thereby impacting gene expression. Additionally, SmcX interacts with iron and α-ketoglutarate as cofactors and can recruit histone deacetylases to neuron silencer elements, effectively repressing transcription of neuronal genes. Defects in the gene encoding SmcX are linked to X-linked mental retardation (XLMR), a condition characterized by cognitive impairment and low IQ, highlighting SmcX′s critical role in neurodevelopment. Multiple isoforms of SmcX arise from alternative splicing events, adding complexity to SmcX′s functional repertoire.

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SmcX Antibody (G-10) References:

1. Mutations in the JARID1C gene, which is involved in transcriptional regulation and chromatin remodeling, cause X-linked mental retardation.  |  Jensen, LR., et al. 2005. Am J Hum Genet. 76: 227-36. PMID: 15586325
2. A novel mutation in JARID1C gene associated with mental retardation.  |  Santos, C., et al. 2006. Eur J Hum Genet. 14: 583-6. PMID: 16538222
3. Novel JARID1C/SMCX mutations in patients with X-linked mental retardation.  |  Tzschach, A., et al. 2006. Hum Mutat. 27: 389. PMID: 16541399
4. The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases.  |  Iwase, S., et al. 2007. Cell. 128: 1077-88. PMID: 17320160
5. The histone H3K4 demethylase SMCX links REST target genes to X-linked mental retardation.  |  Tahiliani, M., et al. 2007. Nature. 447: 601-5. PMID: 17468742
6. Repression of Smad3 activity by histone demethylase SMCX/JARID1C.  |  Kim, TD., et al. 2008. Biochem Biophys Res Commun. 366: 563-7. PMID: 18078810
7. A novel mutation in JARID1C/SMCX in a patient with autism spectrum disorder (ASD).  |  Adegbola, A., et al. 2008. Am J Med Genet A. 146A: 505-11. PMID: 18203167
8. Isolation and characterization of XE169, a novel human gene that escapes X-inactivation.  |  Wu, J., et al. 1994. Hum Mol Genet. 3: 153-60. PMID: 8162017

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SmcX Antibody (G-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SmcX Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SmcX Antibody (G-10) Specifications

Alternate Product Name: SmcX

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1303-1383

Epitope Target: SmcX

Epitope Species: mouse

Product Reactivity/Detection: SmcX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SmcX Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent