SLIRP Antibody (B-12): sc-514508
- SLIRP Antibody (B-12) is a mouse monoclonal IgG2a κ SLIRP antibody provided at 200 µg/ml
- raised against amino acids 1-112 representing full length SLIRP of mouse origin
- SLIRP Antibody (B-12) is recommended for detection of SLIRP of mouse origin by WB, IP, IF and ELISA
- Anti-SLIRP Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514508 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SLIRP Antibody (B-12) for WB applications. These reagents are now offered in bundles with SLIRP Antibody (B-12) (see ordering information below).
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SEE ALSO...
SLIRP Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects SLIRP protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SLIRP antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SLIRP protein, also known as SRA stem-loop-interacting RNA-binding protein, DC50, or C14orf156, is a 109 amino acid mitochondrial protein that primarily localizes to the nucleus and is expressed in various tissues, with the highest levels found in the heart, liver, skeletal muscle, and testis. SLIRP contains a single RNA recognition motif (RRM) domain, which is crucial for its function as a nuclear receptor corepressor; SLIRP binds to the STR7 loop of SRA RNA, thereby repressing SRA-mediated nuclear receptor coactivation. SLIRP has been shown to inhibit the transactivation of several nuclear receptors, including glucocorticoid, androgen, thyroid, and vitamin D receptors, suggesting a role in regulating gene expression and mitochondrial function. SLIRP is encoded on human chromosome 14, a region that encompasses over 700 genes and constitutes nearly 3.5% of the human genome, highlighting potential significance in various biological processes and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SLIRP Antibody (B-12) References:
- Estrogen response element-dependent regulation of transcriptional activation of estrogen receptors alpha and beta by coactivators and corepressors. | Klinge, CM., et al. 2004. J Mol Endocrinol. 33: 387-410. PMID: 15525597
- SLIRP, a small SRA binding protein, is a nuclear receptor corepressor. | Hatchell, EC., et al. 2006. Mol Cell. 22: 657-68. PMID: 16762838
- Gene control by large noncoding RNAs. | Shamovsky, I. and Nudler, E. 2006. Sci STKE. 2006: pe40. PMID: 17018852
- The RNA coregulator SRA, its binding proteins and nuclear receptor signaling activity. | Colley, SM., et al. 2008. IUBMB Life. 60: 159-64. PMID: 18380007
- SRA and its binding partners: an expanding role for RNA-binding coregulators in nuclear receptor-mediated gene regulation. | Colley, SM. and Leedman, PJ. 2009. Crit Rev Biochem Mol Biol. 44: 25-33. PMID: 19280430
- Tamoxifen inhibits inward rectifier K+ 2.x family of inward rectifier channels by interfering with phosphatidylinositol 4,5-bisphosphate-channel interactions. | Ponce-Balbuena, D., et al. 2009. J Pharmacol Exp Ther. 331: 563-73. PMID: 19654266
- Prevention and treatment of cervical cancer in mice using estrogen receptor antagonists. | Chung, SH. and Lambert, PF. 2009. Proc Natl Acad Sci U S A. 106: 19467-72. PMID: 19901334
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLIRP Antibody (B-12) | sc-514508 | 200 µg/ml | $322.00 | |||
SLIRP Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-531149 | 200 µg Ab; 10 µg BP | $361.00 | |||
SLIRP Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-524749 | 200 µg Ab, 40 µg BP | $361.00 | |||
SLIRP Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-547704 | 200 µg Ab; 10 µg BP | $361.00 | |||
SLIRP Antibody (B-12) X | sc-514508 X | 200 µg/0.1 ml | $322.00 | |||
SLIRP Antibody (B-12) AC | sc-514508 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SLIRP Antibody (B-12) HRP | sc-514508 HRP | 200 µg/ml | $322.00 | |||
SLIRP Antibody (B-12) FITC | sc-514508 FITC | 200 µg/ml | $336.00 | |||
SLIRP Antibody (B-12) PE | sc-514508 PE | 200 µg/ml | $349.00 | |||
SLIRP Antibody (B-12) Alexa Fluor® 488 | sc-514508 AF488 | 200 µg/ml | $364.00 | |||
SLIRP Antibody (B-12) Alexa Fluor® 546 | sc-514508 AF546 | 200 µg/ml | $364.00 | |||
SLIRP Antibody (B-12) Alexa Fluor® 594 | sc-514508 AF594 | 200 µg/ml | $364.00 | |||
SLIRP Antibody (B-12) Alexa Fluor® 647 | sc-514508 AF647 | 200 µg/ml | $364.00 | |||
SLIRP Antibody (B-12) Alexa Fluor® 680 | sc-514508 AF680 | 200 µg/ml | $364.00 | |||
SLIRP Antibody (B-12) Alexa Fluor® 790 | sc-514508 AF790 | 200 µg/ml | $364.00 |