SLA/LP Antibody (E-11): sc-514729

SLA/LP Antibody (E-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects SLA/LP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SLA/LP (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SLA/LP protein, also known as SEPSECS (Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase) or SLA-p35, is a 501 amino acid cytoplasmic protein that plays a crucial role in the biosynthesis of selenoproteins, which are essential for various biological processes, including antioxidant defense and redox regulation. Located predominantly in the liver, lung, kidney, and pancreas, SLA/LP is part of a diverse family of pyridoxal phosphate-dependent enzymes that facilitate the aminoacylation of tRNAs. Specifically, SLA/LP catalyzes the conversion of O-phosphoseryl-tRNA(Sec) to Sec-tRNA(Sec) by replacing the phosphate group with a selenol moiety, a critical step in the synthesis of selenocysteine, the 21st amino acid. The importance of this function is underscored by the fact that selenoproteins are involved in critical cellular processes, and their dysfunction can lead to various diseases. Additionally, alternative splicing of the SLA/LP gene results in two distinct isoforms, further highlighting the complexity and regulatory mechanisms governing selenoprotein biosynthesis.

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SLA/LP Antibody (E-11) References:

1. Isolation and characterization of cDNA encoding the antigenic protein of the human tRNP(Ser)Sec complex recognized by autoantibodies from patients withtype-1 autoimmune hepatitis.  |  Costa, M., et al. 2000. Clin Exp Immunol. 121: 364-74. PMID: 10931155
2. Soluble liver antigen: isolation of a 35-kd recombinant protein (SLA-p35) specifically recognizing sera from patients with autoimmune hepatitis.  |  Volkmann, M., et al. 2001. Hepatology. 33: 591-6. PMID: 11230739
3. Evidence for direct roles of two additional factors, SECp43 and soluble liver antigen, in the selenoprotein synthesis machinery.  |  Xu, XM., et al. 2005. J Biol Chem. 280: 41568-75. PMID: 16230358
4. RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea.  |  Yuan, J., et al. 2006. Proc Natl Acad Sci U S A. 103: 18923-7. PMID: 17142313
5. Biosynthesis of selenocysteine on its tRNA in eukaryotes.  |  Xu, XM., et al. 2007. PLoS Biol. 5: e4. PMID: 17194211
6. Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation.  |  Araiso, Y., et al. 2008. Nucleic Acids Res. 36: 1187-99. PMID: 18158303
7. Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation.  |  Sherrer, RL., et al. 2008. Nucleic Acids Res. 36: 1247-59. PMID: 18174226
8. Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase.  |  Sherrer, RL., et al. 2008. Nucleic Acids Res. 36: 1871-80. PMID: 18267971
9. Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.  |  Hauenstein, SI. and Perona, JJ. 2008. J Biol Chem. 283: 22007-17. PMID: 18559341

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SLA/LP Antibody (E-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SLA/LP Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SLA/LP Antibody (E-11) Specifications

Alternate Product Name: SLA/LP

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 360-374

Epitope Target: SLA/LP

Epitope Species: human

Product Reactivity/Detection: SLA/LP isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SLA/LP Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin