Skeletrophin Antibody (D-3): sc-515902
- Skeletrophin Antibody (D-3) is a mouse monoclonal IgG2a κ Skeletrophin antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 92-115 within an internal region of skeletrophin of human origin
- Skeletrophin Antibody (D-3) is recommended for detection of Skeletrophin of human origin by WB, IP, IF and ELISA
- Anti-Skeletrophin Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Skeletrophin Antibody (D-3) for WB applications. These reagents are now offered in bundles with Skeletrophin Antibody (D-3) (see ordering information below).
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Skeletrophin Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Skeletrophin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Skeletrophin antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Skeletrophin, also known as MIB2 (Mindbomb homolog 2), SKD, ZZANK1, or ZZZ5, functions as an E3 ubiquitin-protein ligase that plays a crucial role in regulating Notch signaling through targeted degradation of specific proteins. This regulation is vital for maintaining proper cellular communication and differentiation, particularly in developmental processes and tissue homeostasis. Skeletrophin is predominantly expressed in heart, brain, kidney, and skeletal muscle, where Skeletrophin positively influences Notch signaling by ubiquitinating the intracellular domain of Delta receptors, which serve as ligands for Notch proteins. This ubiquitination process promotes transendocytosis of the Notch extracellular domain, facilitating transport of the Notch intracellular domain to the nucleus, where Skeletrophin activates a range of genes essential for various biological functions. Notably, Skeletrophin localizes to the endosome and exhibits down-regulation in primary skin melanomas, with studies indicating that treatment with 5′-aza-2-deoxycytidine, a demethylating agent, can enhance Skeletrophin expression, suggesting that down-regulation may be linked to methylation of the Skeletrophin gene. Additionally, six isoforms of Skeletrophin exist due to alternative splicing events, further underscoring the complexity and significance of this protein in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Skeletrophin Antibody (D-3) References:
- Down-regulation of a novel actin-binding molecule, skeletrophin, in malignant melanoma. | Takeuchi, T., et al. 2003. Am J Pathol. 163: 1395-404. PMID: 14507647
- Mind bomb-2 is an E3 ligase for Notch ligand. | Koo, BK., et al. 2005. J Biol Chem. 280: 22335-42. PMID: 15824097
- Skeletrophin, a novel RING molecule controlled by the chromatin remodeling complex, is downregulated in malignant melanoma. | Takeuchi, T., et al. 2005. DNA Cell Biol. 24: 339-44. PMID: 15869411
- Skeletrophin, a novel ubiquitin ligase to the intracellular region of Jagged-2, is aberrantly expressed in multiple myeloma. | Takeuchi, T., et al. 2005. Am J Pathol. 166: 1817-26. PMID: 15920166
- A ubiquitin ligase, skeletrophin, is a negative regulator of melanoma invasion. | Takeuchi, T., et al. 2006. Oncogene. 25: 7059-69. PMID: 16715130
- An obligatory role of mind bomb-1 in notch signaling of mammalian development. | Koo, BK., et al. 2007. PLoS One. 2: e1221. PMID: 18043734
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Skeletrophin Antibody (D-3) | sc-515902 | 200 µg/ml | $322.00 | |||
Skeletrophin Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-531555 | 200 µg Ab; 10 µg BP | $361.00 | |||
Skeletrophin Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-525276 | 200 µg Ab, 40 µg BP | $361.00 | |||
Skeletrophin Antibody (D-3): m-IgG2a BP-HRP Bundle | sc-547818 | 200 µg Ab; 10 µg BP | $361.00 | |||
Skeletrophin Antibody (D-3) AC | sc-515902 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Skeletrophin Antibody (D-3) HRP | sc-515902 HRP | 200 µg/ml | $322.00 | |||
Skeletrophin Antibody (D-3) FITC | sc-515902 FITC | 200 µg/ml | $336.00 | |||
Skeletrophin Antibody (D-3) PE | sc-515902 PE | 200 µg/ml | $349.00 | |||
Skeletrophin Antibody (D-3) Alexa Fluor® 488 | sc-515902 AF488 | 200 µg/ml | $364.00 | |||
Skeletrophin Antibody (D-3) Alexa Fluor® 546 | sc-515902 AF546 | 200 µg/ml | $364.00 | |||
Skeletrophin Antibody (D-3) Alexa Fluor® 594 | sc-515902 AF594 | 200 µg/ml | $364.00 | |||
Skeletrophin Antibody (D-3) Alexa Fluor® 647 | sc-515902 AF647 | 200 µg/ml | $364.00 | |||
Skeletrophin Antibody (D-3) Alexa Fluor® 680 | sc-515902 AF680 | 200 µg/ml | $364.00 | |||
Skeletrophin Antibody (D-3) Alexa Fluor® 790 | sc-515902 AF790 | 200 µg/ml | $364.00 |