SIRT4 Antibody (95.1): sc-135797

SIRT4 Antibody (95.1) is a mouse monoclonal IgG2a kappa light chain antibody that detects SIRT4 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-SIRT4 antibody (95.1) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). SIRT4, also known as SIR2L4, is a member of the class II family of sirtuins, which are NAD-dependent deacetylases that play crucial roles in cellular regulation. SIRT4 protein is primarily located in the mitochondrial matrix, where SIRT4 is involved in the regulation of metabolic processes. The mitochondrial localization of SIRT4 is significant because SIRT4 directly influences mitochondrial function and energy metabolism, which are vital for maintaining cellular homeostasis. SIRT4 has been shown to interact with insulin-degrading enzymes, and through ADP-ribosyltransferase activity, SIRT4 negatively regulates insulin secretion from pancreatic β cells, highlighting SIRT4′s importance in glucose metabolism and energy balance. The presence of a deacetylase sirtuin-type domain and the ability to bind zinc as a catalytic cofactor further underscore the functional versatility of SIRT4 in cellular signaling pathways.

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SIRT4 Antibody (95.1) References:

1. Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.  |  Frye, RA. 1999. Biochem Biophys Res Commun. 260: 273-9. PMID: 10381378
2. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.  |  Frye, RA. 2000. Biochem Biophys Res Commun. 273: 793-8. PMID: 10873683
3. Differential regulation of the Sir2 histone deacetylase gene family by inhibitors of class I and II histone deacetylases.  |  Kyrylenko, S., et al. 2003. Cell Mol Life Sci. 60: 1990-7. PMID: 14523559
4. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.  |  Michishita, E., et al. 2005. Mol Biol Cell. 16: 4623-35. PMID: 16079181
5. Histone deacetylases in acute myeloid leukaemia show a distinctive pattern of expression that changes selectively in response to deacetylase inhibitors.  |  Bradbury, CA., et al. 2005. Leukemia. 19: 1751-9. PMID: 16121216
6. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells.  |  Haigis, MC., et al. 2006. Cell. 126: 941-54. PMID: 16959573
7. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase.  |  Ahuja, N., et al. 2007. J Biol Chem. 282: 33583-33592. PMID: 17715127
8. Localization of mouse mitochondrial SIRT proteins: shift of SIRT3 to nucleus by co-expression with SIRT5.  |  Nakamura, Y., et al. 2008. Biochem Biophys Res Commun. 366: 174-9. PMID: 18054327
9. SIRT4 is the last puzzle of mitochondrial sirtuins.  |  Li, Y., et al. 2018. Bioorg Med Chem. 26: 3861-3865. PMID: 30033389
10. Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle.  |  Hu, SH., et al. 2023. Nat Metab. 5: 626-641. PMID: 37081161
11. MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4.  |  Martino, E., et al. 2023. Cell Mol Biol Lett. 28: 66. PMID: 37587410
12. Sirtuin 4 (Sirt4) downregulation contributes to chondrocyte senescence and osteoarthritis via mediating mitochondrial dysfunction.  |  Lin, S., et al. 2024. Int J Biol Sci. 20: 1256-1278. PMID: 38385071

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIRT4 Antibody (95.1) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-SIRT4 Antibody (95.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIRT4 Antibody (95.1) Specifications

Alternate Product Name: SIRT4

Product Clone Name: 95.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 241-255

Epitope Target: SIRT4

Epitope Species: human

Product Reactivity/Detection: SIRT4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for SIRT4 Antibody (95.1): AC: Agarose, HRP: Horseradish Peroxidase