Siglec-1 Antibody (B-1): sc-398109
- Siglec-1 Antibody (B-1) is a mouse monoclonal IgG1 κ Siglec-1 antibody provided at 200 µg/ml
- raised against amino acids 42-97 mapping near the N-terminus of Siglec-1 of human origin
- Siglec-1 Antibody (B-1) is recommended for detection of Siglec-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Siglec-1 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Siglec-1 Antibody (B-1) for WB applications. These reagents are now offered in bundles with Siglec-1 Antibody (B-1) (see ordering information below).
Siglec-1 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Siglec-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Siglec-1 antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Siglec-1, also known as CD169, plays a crucial role in the immune system as a sialic acid-binding receptor expressed on hemopoietic cells, particularly macrophages. Siglec-1 is involved in mediating cell-cell interactions within lymphoid tissues, which is essential for proper functioning of immune responses. Notably, Siglec-1 has a higher affinity for N-acetylneuraminic acid (Neu5Ac) compared to N-glycolylneuraminic acid (Neu5Gc), highlighting specificity in recognizing sialylated ligands. The human Siglec-1 gene is located on chromosome 20p13 and encodes a 1,709 amino acid protein, with alternative splicing resulting in a soluble isoform that is not membrane-bound. This unique structural characteristic allows Siglec-1 to participate in various immunological processes, including regulation of immune cell interactions and modulation of inflammatory responses, making Siglec-1 a significant target for research in immunology and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Siglec-1 Antibody (B-1) References:
- Siglec-1 and -2 as potential biomarkers in autoimmune disease. | Eakin, AJ., et al. 2016. Proteomics Clin Appl. 10: 635-44. PMID: 26752092
- Identification of Siglec-1 null individuals infected with HIV-1. | Martinez-Picado, J., et al. 2016. Nat Commun. 7: 12412. PMID: 27510803
- The role of Siglec-1 in HIV-1/macrophage interaction. | Jobe, O., et al. 2016. Macrophage (Houst). 3: PMID: 28286869
- Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells. | Xie, J., et al. 2018. Vet Res. 49: 67. PMID: 30021620
- Constitutive Siglec-1 expression confers susceptibility to HIV-1 infection of human dendritic cell precursors. | Ruffin, N., et al. 2019. Proc Natl Acad Sci U S A. 116: 21685-21693. PMID: 31591213
- Siglec-1 expression on monocytes is associated with the interferon signature in juvenile dermatomyositis and can predict treatment response. | Lerkvaleekul, B., et al. 2022. Rheumatology (Oxford). 61: 2144-2155. PMID: 34387304
- The role of sialic acid-binding immunoglobulin-like-lectin-1 (siglec-1) in immunology and infectious disease. | Prenzler, S., et al. 2023. Int Rev Immunol. 42: 113-138. PMID: 34494938
- Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells. | Gutiérrez-Martínez, E., et al. 2023. Elife. 12: PMID: 36940134
- A novel subpopulation of monocytes with a strong interferon signature indicated by SIGLEC-1 is present in patients with in recent-onset type 1 diabetes. | Guo, M., et al. 2024. Diabetologia. 67: 623-640. PMID: 38349399
- Identification of Siglec-1-negative alveolar macrophages with proinflammatory phenotypes in chronic obstructive pulmonary disease. | Saito, T., et al. 2024. Am J Physiol Lung Cell Mol Physiol. 326: L672-L686. PMID: 38530936
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Siglec-1 Antibody (B-1) | sc-398109 | 200 µg/ml | $316.00 | |||
Siglec-1 Antibody (B-1): m-IgG Fc BP-HRP Bundle | sc-530692 | 200 µg Ab; 10 µg BP | $354.00 | |||
Siglec-1 Antibody (B-1): m-IgGκ BP-HRP Bundle | sc-524218 | 200 µg Ab, 40 µg BP | $354.00 | |||
Siglec-1 Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-544201 | 200 µg Ab; 20 µg BP | $354.00 | |||
Siglec-1 Antibody (B-1) AC | sc-398109 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Siglec-1 Antibody (B-1) HRP | sc-398109 HRP | 200 µg/ml | $316.00 | |||
Siglec-1 Antibody (B-1) FITC | sc-398109 FITC | 200 µg/ml | $330.00 | |||
Siglec-1 Antibody (B-1) PE | sc-398109 PE | 200 µg/ml | $343.00 | |||
Siglec-1 Antibody (B-1) Alexa Fluor® 488 | sc-398109 AF488 | 200 µg/ml | $357.00 | |||
Siglec-1 Antibody (B-1) Alexa Fluor® 546 | sc-398109 AF546 | 200 µg/ml | $357.00 | |||
Siglec-1 Antibody (B-1) Alexa Fluor® 594 | sc-398109 AF594 | 200 µg/ml | $357.00 | |||
Siglec-1 Antibody (B-1) Alexa Fluor® 647 | sc-398109 AF647 | 200 µg/ml | $357.00 | |||
Siglec-1 Antibody (B-1) Alexa Fluor® 680 | sc-398109 AF680 | 200 µg/ml | $357.00 | |||
Siglec-1 Antibody (B-1) Alexa Fluor® 790 | sc-398109 AF790 | 200 µg/ml | $357.00 |