Siglec-1 Antibody (HSn 7D2): sc-53442

Siglec-1 Antibody (HSn 7D2) is a mouse monoclonal IgG1 kappa light chain antibody that detects human Siglec-1 by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Siglec-1 antibody (HSn 7D2) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Siglec-1, also known as CD169, is a sialic acid-binding receptor predominantly expressed on hemopoietic cells, playing a crucial role in mediating cell-cell interactions within lymphoid tissues. Siglec-1 recognizes sialylated ligands, which are essential for various biological processes, including immune response and cell signaling. The human Siglec-1 gene, located on chromosome 20p13, encodes a 1,709 amino acid protein that can exist in both membrane-bound and soluble forms due to alternative splicing. Siglec-1 exhibits a higher affinity for N-acetylneuraminic acid (Neu5Ac) compared to N-glycolylneuraminic acid (Neu5Gc), which has implications for interactions with different cell types and role in immune regulation. Siglec-1 expression at contact points between macrophages and other cells highlights its significance in facilitating local immune responses and maintaining tissue homeostasis.

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Siglec-1 Antibody (HSn 7D2) References:

1. New aspects of siglec binding specificities, including the significance of fucosylation and of the sialyl-Tn epitope. Sialic acid-binding immunoglobulin superfamily lectins.  |  Brinkman-Van der Linden, EC. and Varki, A. 2000. J Biol Chem. 275: 8625-32. PMID: 10722702
2. Loss of N-glycolylneuraminic acid in human evolution. Implications for sialic acid recognition by siglecs.  |  Brinkman-Van der Linden, EC., et al. 2000. J Biol Chem. 275: 8633-40. PMID: 10722703
3. Ultrastructural localisation of sialoadhesin (siglec-1) on macrophages in rodent lymphoid tissues.  |  Schadee-Eestermans, IL., et al. 2000. Immunobiology. 202: 309-25. PMID: 11131149
4. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.  |  Hartnell, A., et al. 2001. Blood. 97: 288-96. PMID: 11133773
5. Siglec-1 and -2 as potential biomarkers in autoimmune disease.  |  Eakin, AJ., et al. 2016. Proteomics Clin Appl. 10: 635-44. PMID: 26752092
6. Identification of Siglec-1 null individuals infected with HIV-1.  |  Martinez-Picado, J., et al. 2016. Nat Commun. 7: 12412. PMID: 27510803
7. Constitutive Siglec-1 expression confers susceptibility to HIV-1 infection of human dendritic cell precursors.  |  Ruffin, N., et al. 2019. Proc Natl Acad Sci U S A. 116: 21685-21693. PMID: 31591213
8. The role of sialic acid-binding immunoglobulin-like-lectin-1 (siglec-1) in immunology and infectious disease.  |  Prenzler, S., et al. 2023. Int Rev Immunol. 42: 113-138. PMID: 34494938
9. Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells.  |  Gutiérrez-Martínez, E., et al. 2023. Elife. 12: PMID: 36940134
10. A novel subpopulation of monocytes with a strong interferon signature indicated by SIGLEC-1 is present in patients with in recent-onset type 1 diabetes.  |  Guo, M., et al. 2024. Diabetologia. 67: 623-640. PMID: 38349399
11. Identification of Siglec-1-negative alveolar macrophages with proinflammatory phenotypes in chronic obstructive pulmonary disease.  |  Saito, T., et al. 2024. Am J Physiol Lung Cell Mol Physiol. 326: L672-L686. PMID: 38530936
12. The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.  |  Steiniger, B., et al. 1997. Immunology. 92: 307-16. PMID: 9415041

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Siglec-1 Antibody (HSn 7D2) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-Siglec-1 Antibody (HSn 7D2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Siglec-1 Antibody (HSn 7D2) Specifications

Alternate Product Name: Siglec-1

Product Clone Name: HSn 7D2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Siglec-1

Epitope Species: human

Product Reactivity/Detection: Siglec-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Siglec-1 Antibody (HSn 7D2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin