SHPRH Antibody (E-10): sc-514395
- SHPRH Antibody (E-10) is a mouse monoclonal IgG1 κ SHPRH antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 777-806 within an internal region of SHPRH of human origin
- SHPRH Antibody (E-10) is recommended for detection of SHPRH of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SHPRH Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-514395 X, 200 µg/0.1 ml)
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SHPRH Antibody (E-10) for WB applications. This reagent is now offered in a bundle with SHPRH Antibody (E-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SHPRH Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects SHPRH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SHPRH (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SHPRH protein, known as SNF2 histone linker PHD RING helicase, plays a crucial role in DNA repair mechanisms by functioning as an E3 ubiquitin-protein ligase, which is essential for maintaining genomic stability. SHPRH protein is composed of 1,683 amino acids and features a RING-type zinc finger, a PHD-type zinc finger, a Histone H1 domain, and a helicase C-terminal and ATP-binding domain, highlighting its complex structure that is vital for its function. SHPRH is expressed in a wide range of tissues and exists as a homodimer, with human SHPRH sharing 86% amino acid identity with its mouse counterpart, indicating a conserved role across species. SHPRH′s importance in the ubiquitination process, which targets short-lived or abnormal proteins for degradation, underscores its significance in cellular homeostasis and the prevention of diseases related to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SHPRH Antibody (E-10) References:
- Cloning and characterization of a novel gene, SHPRH, encoding a conserved putative protein with SNF2/helicase and PHD-finger domains from the 6q24 region. | Sood, R., et al. 2003. Genomics. 82: 153-61. PMID: 12837266
- Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen. | Unk, I., et al. 2006. Proc Natl Acad Sci U S A. 103: 18107-12. PMID: 17108083
- Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination. | Motegi, A., et al. 2006. J Cell Biol. 175: 703-8. PMID: 17130289
- Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks. | Motegi, A., et al. 2008. Proc Natl Acad Sci U S A. 105: 12411-6. PMID: 18719106
- RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light. | Hishida, T., et al. 2009. Nature. 457: 612-5. PMID: 19079240
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SHPRH Antibody (E-10) | sc-514395 | 200 µg/ml | $316.00 | |||
SHPRH Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-524707 | 200 µg Ab, 40 µg BP | $354.00 | |||
SHPRH Antibody (E-10) X | sc-514395 X | 200 µg/0.1 ml | $316.00 | |||
SHPRH Antibody (E-10) AC | sc-514395 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SHPRH Antibody (E-10) HRP | sc-514395 HRP | 200 µg/ml | $316.00 | |||
SHPRH Antibody (E-10) FITC | sc-514395 FITC | 200 µg/ml | $330.00 | |||
SHPRH Antibody (E-10) PE | sc-514395 PE | 200 µg/ml | $343.00 | |||
SHPRH Antibody (E-10) Alexa Fluor® 488 | sc-514395 AF488 | 200 µg/ml | $357.00 | |||
SHPRH Antibody (E-10) Alexa Fluor® 546 | sc-514395 AF546 | 200 µg/ml | $357.00 | |||
SHPRH Antibody (E-10) Alexa Fluor® 594 | sc-514395 AF594 | 200 µg/ml | $357.00 | |||
SHPRH Antibody (E-10) Alexa Fluor® 647 | sc-514395 AF647 | 200 µg/ml | $357.00 | |||
SHPRH Antibody (E-10) Alexa Fluor® 680 | sc-514395 AF680 | 200 µg/ml | $357.00 | |||
SHPRH Antibody (E-10) Alexa Fluor® 790 | sc-514395 AF790 | 200 µg/ml | $357.00 | |||
SHPRH (E-10) Neutralizing Peptide | sc-514395 P | 100 µg/0.5 ml | $68.00 |