SH2D1A Antibody (A-8): sc-398118
- SH2D1A Antibody (A-8) is a mouse monoclonal IgG1 λ SH2D1A antibody provided at 200 µg/ml
- raised against amino acids 1-128 representing full length SH2D1A of human origin
- SH2D1A Antibody (A-8) is recommended for detection of SH2D1A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SH2D1A Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for SH2D1A Antibody (A-8) for WB applications. This reagent is now offered in a bundle with SH2D1A Antibody (A-8) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
SH2D1A Antibody (A-8) is a mouse monoclonal IgG1 antibody that detects SH2D1A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SH2D1A antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SH2D1A protein, also known as SH2 domain protein 1A, SAP, and CD150/SLAM-associated protein, plays a crucial role in immune signaling by modulating the activity of signaling lymphocyte activation molecules (SLAM) at the interface between T and B cells. Specifically, SH2D1A inhibits the recruitment of the tyrosine phosphatase SHP2 to the phosphorylated cytoplasmic domain of SLAM, thereby influencing T cell expansion during immune responses and promoting the production of interferon-γ. This protein is hydrophilic and consists of 128 amino acids, exhibiting 96% homology with its mouse counterpart in both SH2 and tail domains. SH2D1A is expressed in all major subsets of T cells, including CD4+, CD45RO+, CD45RA+, and CD8+, but is absent in B cells. Additionally, SH2D1A interacts with several cell-surface receptors, including SLAM (CD150), CD84, CD229 (LY9), and CD244 (2B4), through its SH2 domain, highlighting its significance in T cell signaling and immune regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SH2D1A Antibody (A-8) References:
- SH2D1A and SLAM protein expression in human lymphocytes and derived cell lines. | Nagy, N., et al. 2000. Int J Cancer. 88: 439-47. PMID: 11054674
- Characterization of SH2D1A missense mutations identified in X-linked lymphoproliferative disease patients. | Morra, M., et al. 2001. J Biol Chem. 276: 36809-16. PMID: 11477068
- SH2D1A regulates T-dependent humoral autoimmunity. | Hron, JD., et al. 2004. J Exp Med. 200: 261-6. PMID: 15263031
- The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells. | Mikhalap, SV., et al. 2004. Blood. 104: 4063-70. PMID: 15315965
- Defective B cell responses in the absence of SH2D1A. | Morra, M., et al. 2005. Proc Natl Acad Sci U S A. 102: 4819-23. PMID: 15774582
- Fine-tuning of immune responses by SLAM-related receptors. | Bhat, R., et al. 2006. J Leukoc Biol. 79: 417-24. PMID: 16365151
- B cell induction of IL-13 expression in NK cells: role of CD244 and SLAM-associated protein. | Gao, N., et al. 2006. J Immunol. 176: 2758-64. PMID: 16493031
- The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM. | Sayos, J., et al. 1998. Nature. 395: 462-9. PMID: 9774102
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SH2D1A Antibody (A-8) | sc-398118 | 200 µg/ml | $322.00 | |||
SH2D1A Antibody (A-8): m-IgGλ BP-HRP Bundle | sc-524224 | 200 µg Ab, 40 µg BP | $361.00 | |||
SH2D1A Antibody (A-8) AC | sc-398118 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SH2D1A Antibody (A-8) HRP | sc-398118 HRP | 200 µg/ml | $322.00 | |||
SH2D1A Antibody (A-8) FITC | sc-398118 FITC | 200 µg/ml | $336.00 | |||
SH2D1A Antibody (A-8) PE | sc-398118 PE | 200 µg/ml | $349.00 | |||
SH2D1A Antibody (A-8) Alexa Fluor® 488 | sc-398118 AF488 | 200 µg/ml | $364.00 | |||
SH2D1A Antibody (A-8) Alexa Fluor® 546 | sc-398118 AF546 | 200 µg/ml | $364.00 | |||
SH2D1A Antibody (A-8) Alexa Fluor® 594 | sc-398118 AF594 | 200 µg/ml | $364.00 | |||
SH2D1A Antibody (A-8) Alexa Fluor® 647 | sc-398118 AF647 | 200 µg/ml | $364.00 | |||
SH2D1A Antibody (A-8) Alexa Fluor® 680 | sc-398118 AF680 | 200 µg/ml | $364.00 | |||
SH2D1A Antibody (A-8) Alexa Fluor® 790 | sc-398118 AF790 | 200 µg/ml | $364.00 |