SF3B3 Antibody (B-4): sc-398670
- SF3B3 Antibody (B-4) is a mouse monoclonal IgG1 κ SF3B3 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 388-687 mapping within an internal region of SF3B3 of human origin
- SF3B3 Antibody (B-4) is recommended for detection of SF3B3 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- Anti-SF3B3 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SF3B3 Antibody (B-4) for WB applications. These reagents are now offered in bundles with SF3B3 Antibody (B-4) (see ordering information below).
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SF3B3 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SF3B3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SF3B3 (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SF3B3 is a critical component of the U2 small nuclear ribonucleoprotein (snRNP) complex, which is essential for the assembly of the spliceosome, a molecular machine responsible for the splicing of pre-mRNA. This process is vital for proper gene expression, as SF3B3 removes non-coding sequences and joins coding sequences together, ultimately influencing protein diversity and function. Within the spliceosomal complex, SF3B3 interacts with other spliceosomal proteins, including SAP 49, SAP 145, and SAP 155, forming a protein complex that is crucial for the recruitment of U2 snRNP to the branch site of pre-mRNA. Notably, the interaction between SAP 49 and SAP 145 is mediated by the amino-terminus of SAP 49, which contains two RNA-recognition motifs, highlighting the intricate nature of protein-protein interactions that facilitate the splicing process. Anti-SF3B3 antibody (B-4) effectively detects and characterizes SF3B3, making this antibody an invaluable tool for researchers studying gene expression and RNA processing mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SF3B3 Antibody (B-4) References:
- Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. | Krämer, A., et al. 1999. J Cell Biol. 145: 1355-68. PMID: 10385517
- Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155. | Das, BK., et al. 1999. Mol Cell Biol. 19: 6796-802. PMID: 10490618
- The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. | Champion-Arnaud, P. and Reed, R. 1994. Genes Dev. 8: 1974-83. PMID: 7958871
- CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145. | Wells, SE., et al. 1996. Genes Dev. 10: 220-32. PMID: 8566755
- Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits. | Igel, H., et al. 1998. RNA. 4: 1-10. PMID: 9436903
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SF3B3 Antibody (B-4) | sc-398670 | 200 µg/ml | $322.00 | |||
SF3B3 Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-530896 | 200 µg Ab; 10 µg BP | $361.00 | |||
SF3B3 Antibody (B-4): m-IgGκ BP-HRP Bundle | sc-524447 | 200 µg Ab, 40 µg BP | $361.00 | |||
SF3B3 Antibody (B-4): m-IgG1 BP-HRP Bundle | sc-544306 | 200 µg Ab; 20 µg BP | $361.00 | |||
SF3B3 Antibody (B-4) AC | sc-398670 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SF3B3 Antibody (B-4) HRP | sc-398670 HRP | 200 µg/ml | $322.00 | |||
SF3B3 Antibody (B-4) FITC | sc-398670 FITC | 200 µg/ml | $336.00 | |||
SF3B3 Antibody (B-4) PE | sc-398670 PE | 200 µg/ml | $349.00 | |||
SF3B3 Antibody (B-4) Alexa Fluor® 488 | sc-398670 AF488 | 200 µg/ml | $364.00 | |||
SF3B3 Antibody (B-4) Alexa Fluor® 546 | sc-398670 AF546 | 200 µg/ml | $364.00 | |||
SF3B3 Antibody (B-4) Alexa Fluor® 594 | sc-398670 AF594 | 200 µg/ml | $364.00 | |||
SF3B3 Antibody (B-4) Alexa Fluor® 647 | sc-398670 AF647 | 200 µg/ml | $364.00 | |||
SF3B3 Antibody (B-4) Alexa Fluor® 680 | sc-398670 AF680 | 200 µg/ml | $364.00 | |||
SF3B3 Antibody (B-4) Alexa Fluor® 790 | sc-398670 AF790 | 200 µg/ml | $364.00 |