Set1 Antibody (1.4): sc-101858

Set1 Antibody (1.4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Set1 protein of Saccharomyces cerevisiae origin by western blotting (WB) and immunoprecipitation (IP). anti-Set1 antibody (1.4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Set1 protein plays a crucial role in the regulation of gene expression through its function as a histone H3-lysine 4 (H3-K4) methylase, which is essential for maintaining chromatin structure and facilitating DNA repair processes. Set1 protein is part of the COMPASS complex, which includes six other nonessential proteins, and is vital for the regulation of rDNA silencing via H3 methylation. The presence of the highly conserved SET domain within Set1 is significant, as this domain is responsible for enzymatic activity and interaction with other proteins involved in transcriptional regulation. Set1′s association with the phosphorylated form of RNA polymerase II at serine 5 of the C-terminal domain serves as a molecular memory of recent transcriptional activity, highlighting Set1′s importance in the dynamic regulation of gene expression during cellular development.

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Set1 Antibody (1.4) References:

1. The set1Delta mutation unveils a novel signaling pathway relayed by the Rad53-dependent hyperphosphorylation of replication protein A that leads to transcriptional activation of repair genes.  |  Schramke, V., et al. 2001. Genes Dev. 15: 1845-58. PMID: 11459833
2. COMPASS: a complex of proteins associated with a trithorax-related SET domain protein.  |  Miller, T., et al. 2001. Proc Natl Acad Sci U S A. 98: 12902-7. PMID: 11687631
3. Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.  |  Briggs, SD., et al. 2001. Genes Dev. 15: 3286-95. PMID: 11751634
4. A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3.  |  Nagy, PL., et al. 2002. Proc Natl Acad Sci U S A. 99: 90-4. PMID: 11752412
5. COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression.  |  Krogan, NJ., et al. 2002. J Biol Chem. 277: 10753-5. PMID: 11805083
6. Evidence that Set1, a factor required for methylation of histone H3, regulates rDNA silencing in S. cerevisiae by a Sir2-independent mechanism.  |  Bryk, M., et al. 2002. Curr Biol. 12: 165-70. PMID: 11818070
7. Targeted recruitment of Set1 histone methylase by elongating Pol II provides a localized mark and memory of recent transcriptional activity.  |  Ng, HH., et al. 2003. Mol Cell. 11: 709-19. PMID: 12667453
8. The complex activities of the SET1/MLL complex core subunits in development and disease.  |  Jiang, H. 2020. Biochim Biophys Acta Gene Regul Mech. 1863: 194560. PMID: 32302696
9. SET1/MLL family of proteins: functions beyond histone methylation.  |  Sugeedha, J., et al. 2021. Epigenetics. 16: 469-487. PMID: 32795105
10. H3K4me3 regulates RNA polymerase II promoter-proximal pause-release.  |  Wang, H., et al. 2023. Nature. 615: 339-348. PMID: 36859550
11. SET1 and p300 Act Synergistically, through Coupled Histone Modifications, in Transcriptional Activation by p53.  |  Tang, Z., et al. 2023. Cell. 186: 2280. PMID: 37172569
12. SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes.  |  Nislow, C., et al. 1997. Mol Biol Cell. 8: 2421-36. PMID: 9398665

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Set1 Antibody (1.4) has 4 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Set1 Antibody (1.4) Specifications

Alternate Product Name: Set1

Product Clone Name: 1.4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 470-1080

Epitope Target: Set1

Epitope Species: Saccharomyces cerevisiae

Product Reactivity/Detection: Set1

Species Reactivity/Detection: Saccharomyces cerevisiae

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Set1 Antibody (1.4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin