Date published: 2026-4-12

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Septin 3 Antibody (G-6): sc-74431

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Datasheets
  • Septin 3 Antibody (G-6) is a mouse monoclonal IgG2b κ Septin 3 antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 1-50 of Septin 3 of human origin
  • Septin 3 Antibody (G-6) is recommended for detection of Septin 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-Septin 3 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Septin 3 Antibody (G-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Septin 3 Antibody (G-6) (see ordering information below).
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Septin 3 Antibody (G-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Septin 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Septin 3 antibody (G-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Septin 3 plays a crucial role in regulating cellular processes, particularly in cytokinesis and cytoskeleton organization. Septin 3 interacts with other septins and proteins involved in cell division, which is vital for maintaining proper cell function and division. Through forming heteropolymer complexes, Septin 3 participates in various cellular structures, influencing processes such as membrane trafficking and cellular scaffold formation. Septin 3 shows high expression in the brain and undergoes regulation by protein kinase G in neurons, highlighting significance in neurobiology and potential implications in neurodegenerative diseases. Complex septin gene regulation, including alternative splicing, further underscores Septin 3′s importance in diverse biological contexts, making Septin 3 (G-6) monoclonal antibody an essential tool for researchers studying multifaceted roles in cellular dynamics.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Septin 3 Antibody (G-6) References:

  1. Phosphorylation of a new brain-specific septin, G-septin, by cGMP-dependent protein kinase.  |  Xue, J., et al. 2000. J Biol Chem. 275: 10047-56. PMID: 10744683
  2. Reciprocal expression of infant- and adult-preferring transcripts of CDCrel-1 septin gene in the rat neocortex.  |  Toda, S., et al. 2000. Biochem Biophys Res Commun. 273: 723-8. PMID: 10873671
  3. Alternative exon usage of rat septins.  |  Jackisch, BO., et al. 2000. Biochem Biophys Res Commun. 275: 180-8. PMID: 10944462
  4. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif.  |  Larisch, S., et al. 2000. Nat Cell Biol. 2: 915-21. PMID: 11146656
  5. Characterization of the Aspergillus nidulans septin (asp) gene family.  |  Momany, M., et al. 2001. Genetics. 157: 969-77. PMID: 11238387
  6. An ins(X;11)(q24;q23) fuses the MLL and the Septin 6/KIAA0128 gene in an infant with AML-M2.  |  Borkhardt, A., et al. 2001. Genes Chromosomes Cancer. 32: 82-8. PMID: 11477664
  7. Identification of septins in neurofibrillary tangles in Alzheimer's disease.  |  Kinoshita, A., et al. 1998. Am J Pathol. 153: 1551-60. PMID: 9811347

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Septin 3 Antibody (G-6)

sc-74431
200 µg/ml
$322.00

Septin 3 Antibody (G-6): m-IgGκ BP-HRP Bundle

sc-521111
200 µg Ab, 40 µg BP
$361.00

Septin 3 Antibody (G-6): m-IgG2b BP-HRP Bundle

sc-548863
200 µg Ab; 10 µg BP
$361.00

Septin 3 Antibody (G-6) AC

sc-74431 AC
500 µg/ml, 25% agarose
$424.00

Septin 3 Antibody (G-6) HRP

sc-74431 HRP
200 µg/ml
$322.00

Septin 3 Antibody (G-6) FITC

sc-74431 FITC
200 µg/ml
$336.00

Septin 3 Antibody (G-6) PE

sc-74431 PE
200 µg/ml
$349.00

Septin 3 Antibody (G-6) Alexa Fluor® 488

sc-74431 AF488
200 µg/ml
$364.00

Septin 3 Antibody (G-6) Alexa Fluor® 546

sc-74431 AF546
200 µg/ml
$364.00

Septin 3 Antibody (G-6) Alexa Fluor® 594

sc-74431 AF594
200 µg/ml
$364.00

Septin 3 Antibody (G-6) Alexa Fluor® 647

sc-74431 AF647
200 µg/ml
$364.00

Septin 3 Antibody (G-6) Alexa Fluor® 680

sc-74431 AF680
200 µg/ml
$364.00

Septin 3 Antibody (G-6) Alexa Fluor® 790

sc-74431 AF790
200 µg/ml
$364.00

Can Septin 3 (G-6): sc-74431 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, Septin 3 (G-6): sc-74431 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced exceptional immunofluorescence membraneProduced exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
Rated 4 out of 5 by from Good band detected in WB using Jurkat wholeGood band detected in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from CrispCrisp, clean band seen in WB using IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-01-03
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Septin 3 Antibody (G-6) is rated 4.7 out of 5 by 3.
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