SEPHS1 Antibody (F-6): sc-365945

SEPHS1 Antibody (F-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects SEPHS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-SEPHS1 antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SEPHS1, also known as selenophosphate synthetase 1, is a crucial enzyme consisting of 392 amino acids that plays a vital role in the biosynthesis of selenocysteine, an amino acid essential for selenoprotein function. SEPHS1 catalyzes the formation of selenophosphate, the active selenium donor, from selenide, ATP, and water, facilitating selenium incorporation into proteins at specific codons. SEPHS1 function depends on a selenium salvage system that recycles L-selenocysteine, ensuring continuous substrate supply for selenophosphate synthesis. Given selenocysteine′s importance in various biological processes, including antioxidant defense and thyroid hormone metabolism, studying SEPHS1 and related interactions is crucial for understanding selenium′s role in health and disease.

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SEPHS1 Antibody (F-6) References:

1. Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.  |  Tamura, T., et al. 2004. Proc Natl Acad Sci U S A. 101: 16162-7. PMID: 15534230
2. Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans.  |  Saiki, R., et al. 2005. FEBS J. 272: 5606-22. PMID: 16262699
3. p53-Mediated enhancement of radiosensitivity by selenophosphate synthetase 1 overexpression.  |  Chung, HJ., et al. 2006. J Cell Physiol. 209: 131-41. PMID: 16786570
4. The selenoproteome exhibits widely varying, tissue-specific dependence on selenoprotein P for selenium supply.  |  Hoffmann, PR., et al. 2007. Nucleic Acids Res. 35: 3963-73. PMID: 17553827
5. Selenoproteinless animals: selenophosphate synthetase SPS1 functions in a pathway unrelated to selenocysteine biosynthesis.  |  Lobanov, AV., et al. 2008. Protein Sci. 17: 176-82. PMID: 18156471
6. SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.  |  Yang, S., et al. 2021. Exp Hematol Oncol. 10: 17. PMID: 33622411
7. Constitutive Oxidative Stress by SEPHS1 Deficiency Induces Endothelial Cell Dysfunction.  |  Jung, J., et al. 2021. Int J Mol Sci. 22: PMID: 34769076
8. SEPHS1 is dispensable for pluripotency maintenance but indispensable for cardiac differentiation in mouse embryonic stem cells.  |  Qiao, L., et al. 2022. Biochem Biophys Res Commun. 590: 125-131. PMID: 34974300
9. Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis.  |  Kang, D., et al. 2022. Nat Commun. 13: 779. PMID: 35140209
10. SEPHS1: Its evolution, function and roles in development and diseases.  |  Bang, J., et al. 2022. Arch Biochem Biophys. 730: 109426. PMID: 36202216
11. SEPHS1 Gene: A new master key for neurodevelopmental disorders.  |  Ahmed Mohamed, Z., et al. 2024. Clin Chim Acta. 562: 119844. PMID: 38960024
12. Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis.  |  Low, SC., et al. 1995. J Biol Chem. 270: 21659-64. PMID: 7665581

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SEPHS1 Antibody (F-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SEPHS1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SEPHS1 Antibody (F-6) Specifications

Alternate Product Name: SEPHS1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-65

Epitope Target: SEPHS1

Epitope Species: human

Product Reactivity/Detection: SEPHS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SEPHS1 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin