SECp43 Antibody (E-7): sc-377448
- SECp43 Antibody (E-7) is a mouse monoclonal IgG2b κ SECp43 antibody provided at 200 µg/ml
- raised against amino acids 141-260 mapping near the C-terminus of SECp43 of human origin
- SECp43 Antibody (E-7) is recommended for detection of SECp43 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-SECp43 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-377448 X, 200 µg/0.1 ml)
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SECp43 Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with SECp43 Antibody (E-7) (see ordering information below).
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SECp43 Antibody (E-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects SECp43 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-SECp43 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SECp43, also known as tRNA (Sec) selenocysteine 1-associated protein 1 or TRNAU1AP, consists of 287 amino acids and belongs to the RRM TRSPAP protein family. SECp43 is primarily localized in the nucleus, although SECp43 is also found in the cytoplasm, with a notable abundance in the nuclear compartment. SECp43 contains two RNA recognition motif (RRM) domains, which are crucial for RNA binding and processing. These domains feature highly conserved regions, including a six amino acid hydrophobic motif (RNP-2) and an octapeptide motif (RNP-1), which are essential for RNA molecule interactions. SECp43 plays a vital role in the early stages of selenocysteine biosynthesis and tRNA charging, as well as in the later stages of the cotranslational incorporation of selenocysteine into selenoproteins. Additionally, SECp43 may be involved in tRNA methylation and associates with various proteins, including mRNP, SELB, and SBP-2. Two isoforms of SECp43 arise from alternative splicing events, which may contribute to functional diversity in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SECp43 Antibody (E-7) References:
- Identification of a protein component of a mammalian tRNA(Sec) complex implicated in the decoding of UGA as selenocysteine. | Ding, F. and Grabowski, PJ. 1999. RNA. 5: 1561-9. PMID: 10606267
- Protein factors mediating selenoprotein synthesis. | Lescure, A., et al. 2002. Curr Protein Pept Sci. 3: 143-51. PMID: 12370018
- Evolutionarily different RNA motifs and RNA-protein complexes to achieve selenoprotein synthesis. | Krol, A. 2002. Biochimie. 84: 765-74. PMID: 12457564
- Evidence for direct roles of two additional factors, SECp43 and soluble liver antigen, in the selenoprotein synthesis machinery. | Xu, XM., et al. 2005. J Biol Chem. 280: 41568-75. PMID: 16230358
- Supramolecular complexes mediate selenocysteine incorporation in vivo. | Small-Howard, A., et al. 2006. Mol Cell Biol. 26: 2337-46. PMID: 16508009
- The selenoproteome exhibits widely varying, tissue-specific dependence on selenoprotein P for selenium supply. | Hoffmann, PR., et al. 2007. Nucleic Acids Res. 35: 3963-73. PMID: 17553827
- Knowing when not to stop: selenocysteine incorporation in eukaryotes. | Low, SC. and Berry, MJ. 1996. Trends Biochem Sci. 21: 203-8. PMID: 8744353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SECp43 Antibody (E-7) | sc-377448 | 200 µg/ml | $322.00 | |||
SECp43 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-523330 | 200 µg Ab, 40 µg BP | $361.00 | |||
SECp43 Antibody (E-7): m-IgG2b BP-HRP Bundle | sc-549107 | 200 µg Ab; 10 µg BP | $361.00 | |||
SECp43 Antibody (E-7) X | sc-377448 X | 200 µg/0.1 ml | $322.00 | |||
SECp43 Antibody (E-7) AC | sc-377448 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SECp43 Antibody (E-7) HRP | sc-377448 HRP | 200 µg/ml | $322.00 | |||
SECp43 Antibody (E-7) FITC | sc-377448 FITC | 200 µg/ml | $336.00 | |||
SECp43 Antibody (E-7) PE | sc-377448 PE | 200 µg/ml | $349.00 | |||
SECp43 Antibody (E-7) Alexa Fluor® 488 | sc-377448 AF488 | 200 µg/ml | $364.00 | |||
SECp43 Antibody (E-7) Alexa Fluor® 546 | sc-377448 AF546 | 200 µg/ml | $364.00 | |||
SECp43 Antibody (E-7) Alexa Fluor® 594 | sc-377448 AF594 | 200 µg/ml | $364.00 | |||
SECp43 Antibody (E-7) Alexa Fluor® 647 | sc-377448 AF647 | 200 µg/ml | $364.00 | |||
SECp43 Antibody (E-7) Alexa Fluor® 680 | sc-377448 AF680 | 200 µg/ml | $364.00 | |||
SECp43 Antibody (E-7) Alexa Fluor® 790 | sc-377448 AF790 | 200 µg/ml | $364.00 |