Sec63 Antibody (1A8): sc-517139
- Sec63 Antibody (1A8) is a mouse monoclonal IgG2a κ Sec63 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against amino acids 631-728 representing partial length Sec63 of human origin
- recommended for detection of Sec63 of mouse, rat and human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Sec63 Antibody (1A8). This work is in progress.
Sec63 Antibody (1A8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Sec63 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Sec63 antibody (1A8) is available as a non-conjugated monoclonal isotype antibody. Sec63 plays a crucial role in the translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane, a process vital for proper protein maturation and function. Located within the ER, Sec63 is part of the Sec61 complex, which serves as a channel for the translocation of proteins into the ER lumen. This complex is essential for maintaining cellular homeostasis, ensuring proteins are correctly folded and modified before transport to their final destinations. The interaction of Sec63 with other components of the translocation machinery, such as Sec62, highlights its importance in the protein transport process, which is critical for various cellular functions, including secretion, membrane insertion, and organelle targeting. Using Sec63 (1A8) antibody in research can provide valuable insights into the mechanisms of protein translocation and the role of Sec63 in cellular physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sec63 Antibody (1A8) References:
- Mammalian Sec61 is associated with Sec62 and Sec63. | Meyer, HA., et al. 2000. J Biol Chem. 275: 14550-7. PMID: 10799540
- Mutations in SEC63 cause autosomal dominant polycystic liver disease. | Davila, S., et al. 2004. Nat Genet. 36: 575-7. PMID: 15133510
- A protein-conducting channel in the endoplasmic reticulum. | Simon, SM. and Blobel, G. 1991. Cell. 65: 371-80. PMID: 1902142
- Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity. | Fedeles, SV., et al. 2015. J Clin Invest. 125: 1955-67. PMID: 25844898
- Emerging View on the Molecular Functions of Sec62 and Sec63 in Protein Translocation. | Jung, SJ. and Kim, H. 2021. Int J Mol Sci. 22: PMID: 34884562
- Activation of ACLY by SEC63 deploys metabolic reprogramming to facilitate hepatocellular carcinoma metastasis upon endoplasmic reticulum stress. | Hu, C., et al. 2023. J Exp Clin Cancer Res. 42: 108. PMID: 37122003
- Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane. | Görlich, D. and Rapoport, TA. 1993. Cell. 75: 615-30. PMID: 8242738
- Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum. | Scidmore, MA., et al. 1993. Mol Biol Cell. 4: 1145-59. PMID: 8305736
- Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes. | Rapoport, TA., et al. 1996. Annu Rev Biochem. 65: 271-303. PMID: 8811181
- Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. | Hanein, D., et al. 1996. Cell. 87: 721-32. PMID: 8929540
- Identification of a human cDNA homologue to the Drosophila translocation protein 1 (Dtrp1). | Daimon, M., et al. 1997. Biochem Biophys Res Commun. 230: 100-4. PMID: 9020021
- Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex. | Beckmann, R., et al. 1997. Science. 278: 2123-6. PMID: 9405348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec63 Antibody (1A8) | sc-517139 | 100 µg/ml | $316.00 |