Sec61β Antibody (E-6): sc-393633

Sec61β Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Sec61 beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Sec61β antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sec61 beta protein plays a crucial role in the translocation of nascent polypeptides across the endoplasmic reticulum membrane, which is essential for proper protein folding and maturation. This process maintains cellular homeostasis and ensures proteins reach their correct destinations within the cell. The Sec61 complex, which includes Sec61 beta, interacts with Sec62 and Sec63, forming a sophisticated channel that facilitates protein entry into the endoplasmic reticulum and allows retrotranslocation of misfolded proteins back to the cytosol for degradation. This dual functionality highlights Sec61 beta′s importance in both protein synthesis and quality control within the cell, making Sec61 beta a valuable target for research in cellular biology and related fields.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sec61β Antibody (E-6) References:

1. Surfing the Sec61 channel: bidirectional protein translocation across the ER membrane.  |  Römisch, K. 1999. J Cell Sci. 112 (Pt 23): 4185-91. PMID: 10564637
2. Mammalian Sec61 is associated with Sec62 and Sec63.  |  Meyer, HA., et al. 2000. J Biol Chem. 275: 14550-7. PMID: 10799540
3. Role of the cytoplasmic segments of Sec61alpha in the ribosome-binding and translocation-promoting activities of the Sec61 complex.  |  Raden, D., et al. 2000. J Cell Biol. 150: 53-64. PMID: 10893256
4. In vitro binding of ribosomes to the beta subunit of the Sec61p protein translocation complex.  |  Levy, R., et al. 2001. J Biol Chem. 276: 2340-6. PMID: 11036067
5. Ribophorin I associates with a subset of membrane proteins after their integration at the sec61 translocon.  |  Wilson, CM., et al. 2005. J Biol Chem. 280: 4195-206. PMID: 15556939
6. The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum.  |  Alder, NN., et al. 2005. J Cell Biol. 168: 389-99. PMID: 15684029
7. Identification of SEC61β and its autoantibody as biomarkers for colorectal cancer.  |  Fan, CW., et al. 2011. Clin Chim Acta. 412: 887-93. PMID: 21255561
8. Sec61β facilitates the maintenance of endoplasmic reticulum homeostasis by associating microtubules.  |  Zhu, Y., et al. 2018. Protein Cell. 9: 616-628. PMID: 29168059
9. Sec61 channel subunit Sbh1/Sec61β promotes ER translocation of proteins with suboptimal targeting sequences and is fine-tuned by phosphorylation.  |  Barbieri, G., et al. 2023. J Biol Chem. 299: 102895. PMID: 36639027
10. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes.  |  Rapoport, TA., et al. 1996. Annu Rev Biochem. 65: 271-303. PMID: 8811181
11. Identification of a human cDNA homologue to the Drosophila translocation protein 1 (Dtrp1).  |  Daimon, M., et al. 1997. Biochem Biophys Res Commun. 230: 100-4. PMID: 9020021
12. The mechanism underlying cystic fibrosis transmembrane conductance regulator transport from the endoplasmic reticulum to the proteasome includes Sec61beta and a cytosolic, deglycosylated intermediary.  |  Bebök, Z., et al. 1998. J Biol Chem. 273: 29873-8. PMID: 9792704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sec61β Antibody (E-6) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Sec61β Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sec61β Antibody (E-6) Specifications

Alternate Product Name: Sec61β

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 39-58

Epitope Target: Sec61β

Epitope Species: human

Product Reactivity/Detection: Sec61β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Sec61β Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin