SCUBE3 Antibody (H-10): sc-514697

SCUBE3 Antibody (H-10) is a mouse monoclonal IgG1 antibody that detects SCUBE3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SCUBE3, also known as CEGF3, is a novel secreted protein consisting of 993 amino acids that plays a crucial role in bone cell biology, particularly in osteoblast function and differentiation. Expression of SCUBE3 is predominantly observed in osteoblasts, with minimal presence in non-bone tissues, highlighting SCUBE3′s specificity and importance in skeletal development and maintenance. SCUBE3 is characterized by a unique structure, which includes one CUB domain and nine EGF-like domains, allowing participation in various cellular interactions and signaling pathways. Notably, SCUBE3 can form homo-oligomers and hetero-oligomers with SCUBE1, which may influence functional properties and interactions within the bone microenvironment. Additionally, SCUBE3 undergoes post-translational modifications, including C-terminal proteolytic cleavage and N-glycosylation, which can affect stability and activity. The gene encoding SCUBE3 is located on human chromosome 6p21.31, a region linked to Paget′s disease, underscoring potential implications in metabolic bone disorders. With specific reactivity and versatile applications, anti-SCUBE3 antibody (H-10) serves as an essential tool for researchers studying bone biology and related diseases.

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SCUBE3 Antibody (H-10) References:

1. SCUBE3 is an endogenous TGF-β receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer.  |  Wu, YY., et al. 2011. Oncogene. 30: 3682-93. PMID: 21441952
2. Scube3 is expressed in multiple tissues during development but is dispensable for embryonic survival in the mouse.  |  Xavier, GM., et al. 2013. PLoS One. 8: e55274. PMID: 23383134
3. SCUBE3 overexpression predicts poor prognosis in non-small cell lung cancer.  |  Zhao, C., et al. 2013. Biosci Trends. 7: 264-9. PMID: 24390364
4. SCUBE3 (signal peptide-CUB-EGF domain-containing protein 3) modulates fibroblast growth factor signaling during fast muscle development.  |  Tu, CF., et al. 2014. J Biol Chem. 289: 18928-42. PMID: 24849601
5. The Expression of HSPD1, SCUBE3, CXCL14 and Its Relations with the Prognosis in Osteosarcoma.  |  Liang, W., et al. 2015. Cell Biochem Biophys. 73: 763-8. PMID: 27259322
6. Activation of TGF-β1 Pathway by SCUBE3 Regulates TWIST1 Expression and Promotes Breast Cancer Progression.  |  Yang, X., et al. 2020. Cancer Biother Radiopharm. 35: 120-128. PMID: 31742430
7. SCUBE3 Is Likely a Susceptibility Gene for Systemic Lupus Erythematosus for Chinese Populations.  |  Qi, YY., et al. 2020. J Immunol Res. 2020: 8897936. PMID: 33274247
8. SCUBE3 serves as an independent poor prognostic factor in breast cancer.  |  Huo, Q., et al. 2021. Cancer Cell Int. 21: 268. PMID: 34006286
9. DEPDC1B Promotes Melanoma Angiogenesis and Metastasis through Sequestration of Ubiquitin Ligase CDC16 to Stabilize Secreted SCUBE3.  |  Hu, F., et al. 2022. Adv Sci (Weinh). 9: e2105226. PMID: 35088579
10. β-catenin/TCF4-induced SCUBE3 upregulation promotes ovarian cancer development via HIF-1 signaling pathway.  |  Shen, J., et al. 2024. Mol Cell Endocrinol. 582: 112127. PMID: 38109990

The preferred SCUBE3 antibody is SCUBE3 Antibody (G-5): sc-514696.