SCOP Antibody (H-3): sc-390129

SCOP Antibody (H-3) is a mouse monoclonal IgG2a antibody that detects SCOP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SCOP antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SCOP protein, known as the suprachiasmatic nucleus circadian oscillatory protein, plays a crucial role in regulating circadian rhythms and is involved in the dephosphorylation of Akt, a key player in cell survival and apoptosis. By modulating Akt activity, SCOP influences important cellular processes such as apoptosis and tumor suppression, making SCOP vital for understanding cancer biology and potential therapeutic interventions. Notably, SCOP has been shown to negatively regulate K-Ras signaling within membrane rafts, thereby contributing to the regulation of the Ras-MAPK signaling pathway, which is often dysregulated in various cancers. In human embryonic kidney cell lysates, SCOP produces a major protein and a minor protein, with SCOP expression levels being notably lower in certain colon cancer and glioblastoma cell lines that exhibit elevated Akt phosphorylation. Additionally, SCOP is expressed in rat tissues such as the cerebrum, cerebellum, and testis, highlighting SCOP′s diverse roles in different biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SCOP Antibody (H-3) References:

1. SCOP, a novel gene product expressed in a circadian manner in rat suprachiasmatic nucleus.  |  Shimizu, K., et al. 1999. FEBS Lett. 458: 363-9. PMID: 10570941
2. Suprachiasmatic nucleus circadian oscillatory protein, a novel binding partner of K-Ras in the membrane rafts, negatively regulates MAPK pathway.  |  Shimizu, K., et al. 2003. J Biol Chem. 278: 14920-5. PMID: 12594205
3. Ontogeny of circadian clock gene expression in the pineal and the suprachiasmatic nucleus of chick embryo.  |  Okabayashi, N., et al. 2003. Brain Res. 990: 231-4. PMID: 14568350
4. Mechanism of spontaneous firing in dorsomedial suprachiasmatic nucleus neurons.  |  Jackson, AC., et al. 2004. J Neurosci. 24: 7985-98. PMID: 15371499
5. Signaling within the master clock of the brain: localized activation of mitogen-activated protein kinase by gastrin-releasing peptide.  |  Antle, MC., et al. 2005. J Neurosci. 25: 2447-54. PMID: 15758152
6. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth.  |  Gao, T., et al. 2005. Mol Cell. 18: 13-24. PMID: 15808505
7. The in vitro real-time oscillation monitoring system identifies potential entrainment factors for circadian clocks.  |  Nakahata, Y., et al. 2006. BMC Mol Biol. 7: 5. PMID: 16483373
8. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.  |  Nagase, T., et al. 1998. DNA Res. 5: 31-9. PMID: 9628581

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SCOP Antibody (H-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SCOP Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SCOP Antibody (H-3) Specifications

Alternate Product Name: SCOP

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1334-1393

Epitope Target: SCOP

Epitope Species: human

Product Reactivity/Detection: SCOP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SCOP Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin