SCML2 Antibody (H-4): sc-376289
- SCML2 Antibody (H-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 561-825 mapping at the C-terminus of SCML2 of mouse origin
- recommended for detection of SCML2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SCML2 Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with SCML2 Antibody (H-4) (see ordering information below).
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SCML2 Antibody (H-4) is a mouse monoclonal IgG1 antibody that detects SCML2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SCML2, a human homolog of the Drosophila Polycomb protein SCM, plays a crucial role in transcriptional repression and is part of the Polycomb group (PcG) gene family, which is essential for regulating gene expression during embryonic development. SCML2 is predominantly expressed in the placenta, testis, and thymus, highlighting its importance in reproductive and immune functions. Notably, SCML2 undergoes post-translational modifications, such as phosphorylation by ATR or ATM in response to DNA damage, which can influence activity and stability. Additionally, alternative splicing of SCML2 results in two isoforms, allowing for functional diversity that may be critical in various biological contexts. SCML2′s interaction with other proteins within the PcG complex underscores its significance in maintaining cellular identity and regulating developmental processes, making anti-SCML2 antibody (H-4) an invaluable tool for researchers studying gene regulation and chromatin dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SCML2 Antibody (H-4) References:
- USP7 cooperates with SCML2 to regulate the activity of PRC1. | Lecona, E., et al. 2015. Mol Cell Biol. 35: 1157-68. PMID: 25605328
- Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. | Luo, M., et al. 2015. PLoS Genet. 11: e1004954. PMID: 25634095
- SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination. | Hasegawa, K., et al. 2015. Dev Cell. 32: 574-88. PMID: 25703348
- RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. | Adams, SR., et al. 2018. PLoS Genet. 14: e1007233. PMID: 29462142
- Polycomb protein SCML2 facilitates H3K27me3 to establish bivalent domains in the male germline. | Maezawa, S., et al. 2018. Proc Natl Acad Sci U S A. 115: 4957-4962. PMID: 29686098
- SCML2 promotes heterochromatin organization in late spermatogenesis. | Maezawa, S., et al. 2018. J Cell Sci. 131: PMID: 30097555
- A rapidly evolved domain, the SCML2 DNA-binding repeats, contributes to chromatin binding of mouse SCML2†. | Maezawa, S., et al. 2019. Biol Reprod. 100: 409-419. PMID: 30137219
- Polycomb protein SCML2 mediates paternal epigenetic inheritance through sperm chromatin. | Sakashita, A., et al. 2023. Nucleic Acids Res. 51: 6668-6683. PMID: 37283086
- SCML2 contributes to tumor cell resistance to DNA damage through regulating p53 and CHK1 stability. | Peng, Q., et al. 2023. Cell Death Differ. 30: 1849-1867. PMID: 37353627
- Discordant interactions between YAP1 and polycomb group protein SCML2 determine cell fate. | Boston, AM., et al. 2023. iScience. 26: 107964. PMID: 37810219
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCML2 Antibody (H-4) | sc-376289 | 200 µg/ml | $322.00 | |||
SCML2 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-540540 | 200 µg Ab; 10 µg BP | $361.00 | |||
SCML2 Antibody (H-4): m-IgG1 BP-HRP Bundle | sc-542192 | 200 µg Ab; 20 µg BP | $361.00 |