SC Antibody (C-2): sc-374343

SC Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects SC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SC (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SC protein plays a vital role in mucosal immunity, acting as a molecular chaperone for polymeric immunoglobulins, specifically dimeric IgA and pentameric IgM, which are produced and secreted by B cells in the lamina propria beneath the mucosal lining of polarized epithelial cells. SC protein′s function is crucial as SC protein facilitates the transcellular transport of these immunoglobulin polymers across epithelial barriers, ensuring they remain intact and resistant to enzymatic degradation during the transcytosis process. Secretory component (SC) is generated when polymeric immunoglobulin receptors (pIgRs) on the basolateral surface of glandular epithelia associate with these immunoglobulins and undergo proteolytic cleavage. Human SC gene is located on chromosome 1q31-q41 and encodes a 764 amino acid protein that features five units homologous to the variable regions of immunoglobulins, along with a transmembrane region that shares similarities with certain immunoglobulin variable regions. This structural and functional integrity of SC protein is essential for maintaining effective mucosal immunity, highlighting SC protein′s importance in the immune response.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SC Antibody (C-2) References:

1. Increased immunoglobulin A levels in milk by over-expressing the murine polymeric immunoglobulin receptor gene in the mammary gland epithelial cells of transgenic mice.  |  De Groot, N., et al. 2000. Immunology. 101: 218-24. PMID: 11012775
2. Lymphocyte homing and Ig secretion in the murine mammary gland.  |  van der Feltz, MJ., et al. 2001. Scand J Immunol. 54: 292-300. PMID: 11555393
3. The J chain is essential for polymeric Ig receptor-mediated epithelial transport of IgA.  |  Johansen, FE., et al. 2001. J Immunol. 167: 5185-92. PMID: 11673531
4. Lactoferrin and free secretory component of human milk inhibit the adhesion of enteropathogenic Escherichia coli to HeLa cells.  |  de Araújo, AN. and Giugliano, LG. 2001. BMC Microbiol. 1: 25. PMID: 11690544
5. Molecular cloning and exon-intron mapping of the gene encoding human transmembrane secretory component (the poly-Ig receptor).  |  Krajci, P., et al. 1992. Eur J Immunol. 22: 2309-15. PMID: 1355431
6. Polymeric immunoglobulin receptor.  |  Aroeti, B., et al. 1992. Int Rev Cytol. 137B: 157-68. PMID: 1478818
7. Biophysical and Biochemical Characterization of Avian Secretory Component Provides Structural Insights into the Evolution of the Polymeric Ig Receptor.  |  Stadtmueller, BM., et al. 2016. J Immunol. 197: 1408-14. PMID: 27412418
8. Role of secretory component, a secreted glycoprotein, in the specific uptake of IgA dimer by epithelial cells.  |  Kühn, LC. and Kraehenbuhl, JP. 1979. J Biol Chem. 254: 11072-81. PMID: 387764
9. T cell antigen receptors and the immunoglobulin supergene family.  |  Hood, L., et al. 1985. Cell. 40: 225-9. PMID: 3917857
10. Role of J chain and secretory component in receptor-mediated glandular and hepatic transport of immunoglobulins in man.  |  Brandtzaeg, P. 1985. Scand J Immunol. 22: 111-46. PMID: 3929375
11. Secretory component in immmunoglobulin deficiency: and immunoelectron microscopic study of intestinal epithelium.  |  Nagura, H., et al. 1980. Scand J Immunol. 12: 359-63. PMID: 7466325
12. Secretory component of immunoglobulin A in maternal serum and the prediction of preterm delivery.  |  Schellenberg, JC., et al. 1998. Am J Obstet Gynecol. 178: 535-9. PMID: 9539522

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SC Antibody (C-2) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-SC Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SC Antibody (C-2) Specifications

Alternate Product Name: SC

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 465-764

Epitope Target: SC

Epitope Species: human

Product Reactivity/Detection: SC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for SC Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin