SAP 18 Antibody (C-3): sc-365377
- SAP 18 Antibody (C-3) is a mouse monoclonal IgG2b κ SAP 18 antibody provided at 200 µg/ml
- raised against amino acids 1-130 of SAP18 of human origin
- SAP 18 Antibody (C-3) is recommended for detection of SAP 18 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SAP 18 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-365377 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SAP 18 Antibody (C-3) for WB applications. These reagents are now offered in bundles with SAP 18 Antibody (C-3) (see ordering information below).
SAP 18 Antibody (C-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects SAP 18 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAP 18 (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAP 18 protein plays a crucial role in chromatin remodeling, which is essential for the regulation of gene expression. SAP 18 is part of the mSin3 corepressor complex, which also includes histone deacetylases HDAC1 and HDAC2, and is involved in the deacetylation of histones, leading to transcriptional silencing. This process is vital for maintaining cellular homeostasis and regulating developmental processes, as improper chromatin remodeling can result in aberrant gene expression and contribute to various diseases, including cancer. Using anti-SAP 18 antibody (C-3) in research can provide insights into the mechanisms of transcriptional regulation and the role of chromatin dynamics in cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SAP 18 Antibody (C-3) References:
- The major dermatomyositis-specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation. | Seelig, HP., et al. 1995. Arthritis Rheum. 38: 1389-99. PMID: 7575689
- Nucleosome structural changes due to acetylation. | Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
- A positive role for histone acetylation in transcription factor access to nucleosomal DNA. | Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
- Transcriptional silencing in yeast is associated with reduced nucleosome acetylation. | Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
- Repression and activation by multiprotein complexes that alter chromatin structure. | Kingston, RE., et al. 1996. Genes Dev. 10: 905-20. PMID: 8608939
- Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex. | Zhang, Y., et al. 1997. Cell. 89: 357-64. PMID: 9150135
- SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex. | Zhang, Y., et al. 1998. Mol Cell. 1: 1021-31. PMID: 9651585
- The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities. | Zhang, Y., et al. 1998. Cell. 95: 279-89. PMID: 9790534
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP 18 Antibody (C-3) | sc-365377 | 200 µg/ml | $322.00 | |||
SAP 18 Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-529364 | 200 µg Ab; 10 µg BP | $361.00 | |||
SAP 18 Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-522228 | 200 µg Ab, 40 µg BP | $361.00 | |||
SAP 18 Antibody (C-3): m-IgG2b BP-HRP Bundle | sc-550098 | 200 µg Ab; 10 µg BP | $361.00 | |||
SAP 18 Antibody (C-3) X | sc-365377 X | 200 µg/0.1 ml | $322.00 | |||
SAP 18 Antibody (C-3) AC | sc-365377 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SAP 18 Antibody (C-3) HRP | sc-365377 HRP | 200 µg/ml | $322.00 | |||
SAP 18 Antibody (C-3) FITC | sc-365377 FITC | 200 µg/ml | $336.00 | |||
SAP 18 Antibody (C-3) PE | sc-365377 PE | 200 µg/ml | $349.00 | |||
SAP 18 Antibody (C-3) Alexa Fluor® 488 | sc-365377 AF488 | 200 µg/ml | $364.00 | |||
SAP 18 Antibody (C-3) Alexa Fluor® 546 | sc-365377 AF546 | 200 µg/ml | $364.00 | |||
SAP 18 Antibody (C-3) Alexa Fluor® 594 | sc-365377 AF594 | 200 µg/ml | $364.00 | |||
SAP 18 Antibody (C-3) Alexa Fluor® 647 | sc-365377 AF647 | 200 µg/ml | $364.00 | |||
SAP 18 Antibody (C-3) Alexa Fluor® 680 | sc-365377 AF680 | 200 µg/ml | $364.00 | |||
SAP 18 Antibody (C-3) Alexa Fluor® 790 | sc-365377 AF790 | 200 µg/ml | $364.00 |