SAHH Antibody (A-6): sc-393129

SAHH Antibody (A-6) is a mouse monoclonal IgG1 antibody that detects S-adenosylhomocysteine hydrolase (SAHH) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAHH is a crucial cytoplasmic protein composed of 432 amino acids, primarily localized in the melanosome, an organelle responsible for melanin production. SAHH (A-6) antibody recognizes a protein that plays a vital role in amino acid biosynthesis by catalyzing the reversible hydrolysis of S-adenosylhomocysteine (SAH) into adenosine and L-homocysteine, thereby regulating intracellular SAH levels and influencing methyltransferase reactions. The homotetrameric structure of SAHH allows binding of one NAD molecule per subunit, facilitating involvement in the activated methyl cycle, where the methyl group of methionine is activated through the formation of S-adenosylmethionine. Mutations in the gene encoding SAHH can lead to hypermethioninemia, a metabolic disorder characterized by elevated methionine levels, which can result in symptoms such as sluggishness, muscle weakness, and liver dysfunction. Anti-SAHH antibody (A-6) serves as an essential tool for researchers studying the functional dynamics of SAHH and its implications in various biological processes and diseases.

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SAHH Antibody (A-6) References:

1. Homocysteine-mediated expression of SAHH, DNMTs, MBD2, and DNA hypomethylation potential pathogenic mechanism in VSMCs.  |  Yideng, J., et al. 2007. DNA Cell Biol. 26: 603-11. PMID: 17688412
2. Suppression of methylation-mediated transcriptional gene silencing by βC1-SAHH protein interaction during geminivirus-betasatellite infection.  |  Yang, X., et al. 2011. PLoS Pathog. 7: e1002329. PMID: 22028660
3. Heterologous expression of sahH reveals that biofilm formation is autoinducer-2-independent in Streptococcus sanguinis but is associated with an intact activated methionine cycle.  |  Redanz, S., et al. 2012. J Biol Chem. 287: 36111-22. PMID: 22942290
4. Effects of the crinivirus coat protein-interacting plant protein SAHH on post-transcriptional RNA silencing and its suppression.  |  Cañizares, MC., et al. 2013. Mol Plant Microbe Interact. 26: 1004-15. PMID: 23697374
5. Therapeutic effects of DZ2002, a reversible SAHH inhibitor, on lupus-prone NZB×NZW F1 mice via interference with TLR-mediated APC response.  |  He, SJ., et al. 2014. Acta Pharmacol Sin. 35: 219-29. PMID: 24374810
6. Mycobacterium tuberculosis AtsG (Rv0296c), GlmU (Rv1018c) and SahH (Rv3248c) Proteins Function as the Human IL-8-Binding Effectors and Contribute to Pathogen Entry into Human Neutrophils.  |  Dziadek, B., et al. 2016. PLoS One. 11: e0148030. PMID: 26829648
7. Metformin alters DNA methylation genome-wide via the H19/SAHH axis.  |  Zhong, T., et al. 2017. Oncogene. 36: 2345-2354. PMID: 27775072
8. Topical administration of reversible SAHH inhibitor ameliorates imiquimod-induced psoriasis-like skin lesions in mice via suppression of TNF-α/IFN-γ-induced inflammatory response in keratinocytes and T cell-derived IL-17.  |  Lin, ZM., et al. 2018. Pharmacol Res. 129: 443-452. PMID: 29155016
9. Assay development for determination of DZ2002, a new reversible SAHH inhibitor, and its acid metabolite DZA in blood and application to rat pharmacokinetic study.  |  Jia, W., et al. 2019. J Pharm Anal. 9: 25-33. PMID: 30740254
10. Molecular cloning, characterization and expression analysis of S- adenosyl- L-homocysteine hydrolase (SAHH) during the pathogenic infection of Litopenaeus vannamei by Vibrio alginolyticus.  |  Liang, Q., et al. 2019. Fish Shellfish Immunol. 88: 284-292. PMID: 30849500

The preferred SAHH antibody is SAHH Antibody (A-11): sc-271389.