SAHH Antibody (A-11): sc-271389

SAHH Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SAHH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SAHH (A-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAHH protein, also known as S-adenosylhomocysteine hydrolase or AHCY, is a crucial 432 amino acid cytoplasmic protein that plays a significant role in amino acid biosynthesis by catalyzing the reversible hydrolysis of S-adenosylhomocysteine (SAH) into adenosine and L-homocysteine. This enzymatic activity is vital for regulating intracellular SAH levels, which in turn influences methyltransferase reactions that are essential for various biological processes, including gene expression and protein function. SAHH is structured as a homotetramer, with each subunit capable of binding one molecule of NAD, and is integral to the activated methyl cycle, a pathway that activates the methyl group of methionine through the formation of S-adenosylmethionine. Importantly, defects in the gene encoding SAHH can lead to hypermethioninemia, a metabolic disorder characterized by elevated methionine levels, resulting in symptoms such as sluggishness, muscle weakness, and liver dysfunction.

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SAHH Antibody (A-11) References:

1. Contributions of active site residues to the partial and overall catalytic activities of human S-adenosylhomocysteine hydrolase.  |  Elrod, P., et al. 2002. Biochemistry. 41: 8134-42. PMID: 12069606
2. Catalytic strategy of S-adenosyl-L-homocysteine hydrolase: transition-state stabilization and the avoidance of abortive reactions.  |  Yang, X., et al. 2003. Biochemistry. 42: 1900-9. PMID: 12590576
3. S-Adenosylhomocysteine hydrolase as a target for intracellular adenosine action.  |  Kloor, D. and Osswald, H. 2004. Trends Pharmacol Sci. 25: 294-7. PMID: 15165742
4. Role of S-adenosylhomocysteine hydrolase in adenosine-induced apoptosis in HepG2 cells.  |  Hermes, M., et al. 2007. Exp Cell Res. 313: 264-83. PMID: 17097637
5. S-adenosylhomocysteine hydrolase is localized at the front of chemotaxing cells, suggesting a role for transmethylation during migration.  |  Shu, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 19788-93. PMID: 17172447
6. Comparative kinetics of cofactor association and dissociation for the human and trypanosomal S-adenosylhomocysteine hydrolases. 1. Basic features of the association and dissociation processes.  |  Li, QS., et al. 2007. Biochemistry. 46: 5798-809. PMID: 17447732
7. Homocysteine-mediated expression of SAHH, DNMTs, MBD2, and DNA hypomethylation potential pathogenic mechanism in VSMCs.  |  Yideng, J., et al. 2007. DNA Cell Biol. 26: 603-11. PMID: 17688412
8. Copper ions inhibit S-adenosylhomocysteine hydrolase by causing dissociation of NAD+ cofactor.  |  Li, M., et al. 2007. Biochemistry. 46: 11451-8. PMID: 17892301
9. Epigenetic regulation of TXNIP-mediated oxidative stress and NLRP3 inflammasome activation contributes to SAHH inhibition-aggravated diabetic nephropathy.  |  Dai, X., et al. 2021. Redox Biol. 45: 102033. PMID: 34119876
10. Effects of H19/SAHH/DNMT1 on the oxidative DNA damage related to benzo[a]pyrene exposure.  |  Fu, Y., et al. 2023. Environ Sci Pollut Res Int. 30: 11706-11718. PMID: 36098921
11. Reversible SAHH inhibitor ameliorates MIA-induced osteoarthritis of rats through suppressing MEK/ERK pathway.  |  Fan, SH., et al. 2024. Biomed Pharmacother. 170: 115975. PMID: 38070246
12. Ultrasound-targeted microbubble technology facilitates SAHH gene delivery to treat diabetic cardiomyopathy by activating AMPK pathway.  |  Guo, X., et al. 2024. iScience. 27: 108852. PMID: 38303706

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SAHH Antibody (A-11) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-SAHH Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SAHH Antibody (A-11) Specifications

Alternate Product Name: SAHH

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 296-333

Epitope Target: SAHH

Epitope Species: human

Product Reactivity/Detection: SAHH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for SAHH Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin