Sacsin Antibody (G-3): sc-515118

Sacsin Antibody (G-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects Sacsin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sacsin (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The SACS gene encodes Sacsin protein, which is crucial for chaperone-mediated protein folding, a process essential for maintaining cellular protein homeostasis and preventing the aggregation of misfolded proteins. Sacsin is predominantly expressed in the central nervous system, as well as in skeletal muscle and pancreatic tissues, highlighting its importance in both neuronal and muscular function. Sacsin′s structure includes seven nuclear localization signals, three coiled-coil domains, and two leucine zipper motifs, along with a DnaJ motif and a hydrophobic domain in its C-terminal region, which are vital for its interaction with other proteins and its localization within the cell. Defects in the SACS gene can lead to autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset symptoms such as reduced motor-nerve velocity and hypermyelination of retinal-nerve fibers, particularly prevalent in the Charlevoix-Saguenay-Lac-Saint-Jean region of Quebec.

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Sacsin Antibody (G-3) References:

1. Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS): high-resolution physical and transcript map of the candidate region in chromosome region 13q11.  |  Engert, JC., et al. 1999. Genomics. 62: 156-64. PMID: 10610707
2. Sacsin-related autosomal recessive ataxia without prominent retinal myelinated fibers in Japan.  |  Hara, K., et al. 2005. Mov Disord. 20: 380-2. PMID: 15486997
3. A phenotype without spasticity in sacsin-related ataxia.  |  Shimazaki, H., et al. 2005. Neurology. 64: 2129-31. PMID: 15985586
4. Novel compound heterozygous mutations in sacsin-related ataxia.  |  Yamamoto, Y., et al. 2005. J Neurol Sci. 239: 101-4. PMID: 16198375
5. A novel sacsin mutation in a Japanese woman showing clinical uniformity of autosomal recessive spastic ataxia of Charlevoix-Saguenay.  |  Okawa, S., et al. 2006. J Neurol Neurosurg Psychiatry. 77: 280-2. PMID: 16421146
6. Sacsin-related ataxia caused by the novel nonsense mutation Arg4325X.  |  Yamamoto, Y., et al. 2006. J Neurol. 253: 1372-3. PMID: 16944349
7. The ataxia protein sacsin is a functional co-chaperone that protects against polyglutamine-expanded ataxin-1.  |  Parfitt, DA., et al. 2009. Hum Mol Genet. 18: 1556-65. PMID: 19208651
8. The neurodegenerative-disease-related protein sacsin is a molecular chaperone.  |  Anderson, JF., et al. 2011. J Mol Biol. 411: 870-80. PMID: 21726565
9. Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.  |  Ménade, M., et al. 2018. J Biol Chem. 293: 12832-12842. PMID: 29945973
10. Functional Transcriptome Analysis in ARSACS KO Cell Model Reveals a Role of Sacsin in Autophagy.  |  Morani, F., et al. 2019. Sci Rep. 9: 11878. PMID: 31417125
11. Proteomics and lipidomic analysis reveal dysregulated pathways associated with loss of sacsin.  |  Galatolo, D., et al. 2024. Front Neurosci. 18: 1375299. PMID: 38911600
12. Modeling sacsin depletion in Danio Rerio offers new insight on retinal defects in ARSACS.  |  Naef, V., et al. 2025. Neurobiol Dis. 205: 106793. PMID: 39778749

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sacsin Antibody (G-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Sacsin Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sacsin Antibody (G-3) Specifications

Alternate Product Name: Sacsin

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 787-808

Epitope Target: Sacsin

Epitope Species: human

Product Reactivity/Detection: Sacsin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Sacsin Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin