Sab Antibody (A-3): sc-390512

Sab Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sab protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sab (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sab protein plays a critical role as a Src homology 3 domain (SH3) binding protein, primarily associating with Bruton′s tyrosine kinase (Btk), which is essential for B cell development in both humans and mice. Btk is a member of the Btk/Tec family of cytoplasmic tyrosine kinases and is specifically crucial for B cell maturation; Btk deficiency leads to severe immunodeficiencies such as X-linked agammaglobulinemia in humans and X-linked immunodeficiency in mice. Sab acts as a negative regulator of Btk activity, and Sab binding to Btk not only reduces phosphorylation of Btk substrates but also inhibits Btk-induced auto-phosphorylation in B cells, thereby modulating immune responses. The interaction between SH3 domains of Sab and Btk is vital for this regulatory function, highlighting Sab′s importance in maintaining kinase activity balance within the immune system. Sab′s broader expression compared to Btk suggests potential interactions with additional protein kinases across various tissues, indicating possible roles in diverse cellular processes.

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Sab Antibody (A-3) References:

1. Functions of Bruton's tyrosine kinase in mast and B cells.  |  Kawakami, Y., et al. 1999. J Leukoc Biol. 65: 286-90. PMID: 10080529
2. Bruton's tyrosine kinase activity is negatively regulated by Sab, the Btk-SH3 domain-binding protein.  |  Yamadori, T., et al. 1999. Proc Natl Acad Sci U S A. 96: 6341-6. PMID: 10339589
3. The SH3 domain of Bruton's tyrosine kinase displays altered ligand binding properties when auto-phosphorylated in vitro.  |  Morrogh, LM., et al. 1999. Eur J Immunol. 29: 2269-79. PMID: 10427990
4. Identification of the SH2 domain binding protein of Bruton's tyrosine kinase as BLNK--functional significance of Btk-SH2 domain in B-cell antigen receptor-coupled calcium signaling.  |  Hashimoto, S., et al. 1999. Blood. 94: 2357-64. PMID: 10498607
5. C1q-fixing human leukocyte antigen assay in immunized renal patients: correlation between Luminex SAB-C1q and SAB-IgG.  |  Llorente, S., et al. 2012. Transplant Proc. 44: 2535-7. PMID: 23146446
6. Clinical anatomy of the subacromial and related shoulder bursae: A review of the literature.  |  Kennedy, MS., et al. 2017. Clin Anat. 30: 213-226. PMID: 28033656
7. A case study on Staphylococcus aureus bacteraemia: available treatment options, antibiotic R&D and responsible antibiotic-use strategies.  |  Monnier, AA., et al. 2020. JAC Antimicrob Resist. 2: dlaa034. PMID: 34222996
8. Clinical Risk Factors for Infective Endocarditis Patients With Staphylococcus Aureus Bacteremia and the Diagnostic Utility of Transesophageal Echocardiogram.  |  Khan, UA., et al. 2022. Curr Probl Cardiol. 47: 101331. PMID: 35870547
9. The application of podocyte antigen PLA2R and anti-PLA2R antibody in the diagnosis and treatment of membranous nephropathy.  |  Chen, Y., et al. 2023. Ren Fail. 45: 2264939. PMID: 37814415
10. Phosphorylation of two regulatory tyrosine residues in the activation of Bruton's tyrosine kinase via alternative receptors.  |  Wahl, MI., et al. 1997. Proc Natl Acad Sci U S A. 94: 11526-33. PMID: 9326643
11. Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (BtK).  |  Matsushita, M., et al. 1998. Biochem Biophys Res Commun. 245: 337-43. PMID: 9571151
12. Btk function in B cell development and response.  |  Satterthwaite, AB., et al. 1998. Semin Immunol. 10: 309-16. PMID: 9695187

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sab Antibody (A-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Sab Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sab Antibody (A-3) Specifications

Alternate Product Name: Sab

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 45-157

Epitope Target: Sab

Epitope Species: human

Product Reactivity/Detection: Sab

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Sab Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin