RUNX1 Antibody (A-2): sc-365644

RUNX1 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects RUNX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-RUNX1 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RUNX1, also known as AML1 or CBFA2, plays a critical role in hematopoiesis, the process of blood cell formation, and is essential for proper development of various blood cell lineages. RUNX1 regulates the expression of genes involved in cellular differentiation and cell cycle progression, making RUNX1 a key player in maintaining normal hematopoietic function. RUNX1 is frequently implicated in human leukemia due to chromosomal translocations that disrupt DNA-binding domain, leading to aberrant gene expression and oncogenesis. Beyond hematopoiesis, RUNX1 is crucial for sensory neuron diversification, promoting axonal growth and mediating TrkA transactivation in neural crest-derived sensory neurons. Alternative splicing of RUNX1 results in several isoforms, further diversifying functional roles in cellular processes.

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RUNX1 Antibody (A-2) References:

1. Oncogenic potential of the RUNX gene family: 'overview'.  |  Ito, Y. 2004. Oncogene. 23: 4198-208. PMID: 15156173
2. Role of RUNX family members in transcriptional repression and gene silencing.  |  Durst, KL. and Hiebert, SW. 2004. Oncogene. 23: 4220-4. PMID: 15156176
3. Leukaemia/Drosophila homology.  |  Daga, A., et al. 1992. Nature. 356: 484. PMID: 1560822
4. Role of Runx proteins in chondrogenesis.  |  Yoshida, CA. and Komori, T. 2005. Crit Rev Eukaryot Gene Expr. 15: 243-54. PMID: 16390320
5. The Runx1/AML1 transcription factor selectively regulates development and survival of TrkA nociceptive sensory neurons.  |  Marmigère, F., et al. 2006. Nat Neurosci. 9: 180-7. PMID: 16429136
6. A role for Runx transcription factor signaling in dorsal root ganglion sensory neuron diversification.  |  Kramer, I., et al. 2006. Neuron. 49: 379-93. PMID: 16446142
7. Runx1 Structure and Function in Blood Cell Development.  |  Bonifer, C., et al. 2017. Adv Exp Med Biol. 962: 65-81. PMID: 28299651
8. Role of histone deacetylases in acute leukemia.  |  Fenrick, R. and Hiebert, SW. 1998. J Cell Biochem. 72 Suppl 30-31: 194-202. PMID: 29345812
9. Alternative splicing and genomic structure of the AML1 gene involved in acute myeloid leukemia.  |  Miyoshi, H., et al. 1995. Nucleic Acids Res. 23: 2762-9. PMID: 7651838
10. Fusion of the TEL gene on 12p13 to the AML1 gene on 21q22 in acute lymphoblastic leukemia.  |  Golub, TR., et al. 1995. Proc Natl Acad Sci U S A. 92: 4917-21. PMID: 7761424
11. A large variety of alternatively spliced and differentially expressed mRNAs are encoded by the human acute myeloid leukemia gene AML1.  |  Levanon, D., et al. 1996. DNA Cell Biol. 15: 175-85. PMID: 8634147
12. Leukemogenesis by the chromosomal translocations.  |  Mitani, K. 1997. Leukemia. 11 Suppl 3: 294-6. PMID: 9209370

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RUNX1 Antibody (A-2) has 54 citations in a variety of scientific publications.

Primary antibodies like Anti-RUNX1 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RUNX1 Antibody (A-2) Specifications

Alternate Product Name: RUNX1

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 186-250

Epitope Target: RUNX1

Epitope Species: human

Product Reactivity/Detection: a broad range of RUNX1 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 54 citations in various scientific publications

Available Conjugates/Forms for RUNX1 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent