RUNX1 Antibody (A-2) recommended for detection of a broad range of RUNX1 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

RUNX1 Antibody (A-2): sc-365644

RUNX1 Antibody (A-2) is rated 4.9 out of 5 by 17.
  • y_2020, m_11, d_30, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_365644, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 163ms
  • REVIEWS, PRODUCT
5
15
4
2
3
0
2
0
1
0

Datasheets

    • Anti-RUNX1 Antibody (A-2) is a mouse monoclonal IgG1 κ RUNX1 antibody, cited in 23 publications, provided at 200 µg/ml
    • raised against amino acids 186-250 mapping within an internal region of RUNX1 of human origin
    • Anti-RUNX1 Antibody (A-2) is recommended for detection of a broad range of RUNX1 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-RUNX1 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-365644 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RUNX1 (A-2): sc-365644.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for RUNX1 Antibody (A-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with RUNX1 Antibody (A-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Hello! What is the principle difference between sc-365644 and sc-365644 X? Is sc-365644 X validated for ChIP?

Asked by: Sveta K
Thank you for your question the TransCruz Reagent, sc-365644 X, is 10X more concentrated than the standard formulation of the antibody, in a buffer of PBS + azide (without gelatin). We are aware of one publication citing use of this antibody for ChIP. Please refer to PMID: 29779944 for more details.
Answered by: Technical Support
Date published: 2019-01-06

Is this region present in RUNX2 and RUNX3? Does this antibody cross-react with RUNX2 and 3?

Asked by: BrianE
Thank you for your question. This antibody does not have any cross-reactivity to RUNX2 and RUNX3.
Answered by: Tech Service
Date published: 2019-01-04

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RUNX1 Antibody (A-2): sc-365644?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-09
  • y_2020, m_11, d_28, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_365644, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 134ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Great antibody I have successfully used RUNX1 to stain mouse xenograft of human gastric cancer MKN45 cell line, which I knew to be positive. Plus, it worked also well when staining human gastric carcinoma samples by IHC (PMID: 30662000).
Date published: 2020-06-23
Rated 5 out of 5 by from Anticuerpo excelente para IHC Este anticuerpo detecta específicamente RUNX1 en núcleos de tejidos parafinados y en células fijadas con formaldehído. Muy buen anticuerpo.
Date published: 2018-02-07
Rated 5 out of 5 by from IHC/P Work well in the IHC(P) for tibia of mouse (1:100)
Date published: 2017-10-23
Rated 5 out of 5 by from good quality I used RUNX1 antibody to test HeLa nuclear extract for purity, I have used the antibody for western blotting (1:400) and in for immunofluorescence . It work very well and is very sensitive for western blot . IF Staining also looks correct.
Date published: 2017-08-18
Rated 4 out of 5 by from Good antibody The antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
Rated 5 out of 5 by from its function is excellant I am glad to apply for the sample sack and found that it is very good.
Date published: 2017-07-11
Rated 5 out of 5 by from Western blot Used for analysis rat kidney tissue section slides, 1:300, expected staining.
Date published: 2017-07-08
Rated 5 out of 5 by from work well in 293T and Hela cell in 1:1000 dilution This antibody give very clear band in 293T and Hela cell. I used 10% of whole cell lysate of one well from 6-well plate.
Date published: 2017-05-13
  • y_2020, m_11, d_30, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_365644, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.